MPD: Data set: Rusyn6

Yoo HS, Bradford BU, Kosyk O, Shymonyak S, Uehara T, Collins LB, Bodnar WM, Ball LM, Gold A, Rusyn I. Comparative analysis of the relationship between trichloroethylene metabolism and tissue-specific toxicity among inbred mouse strains: liver effects. J Toxicol Environ Health A. 2015;78(1):15-3doi: 10.1080/15287394.2015.958417. PubMed 25424544 FullText

Yoo HS, Bradford BU, Kosyk O, Uehara T, Shymonyak S, Collins LB, Bodnar WM, Ball LM, Gold A, Rusyn I. Comparative analysis of the relationship between trichloroethylene metabolism and tissue-specific toxicity among inbred mouse strains: kidney effects. J Toxicol Environ Health A. 2015;78(1):32-49. doi: 10.1080/15287394.2015.958418. PubMed 25424545 FullText

Rusyn6 downloads
• Download Rusyn6 project data set animal data, as uploaded
• Download Rusyn6 animal data matrix with factor-related expansion applied as necessary
• Download Rusyn6 strain means, SD, N, etc. one row per strain/sex/measure
• Rusyn6 supplementary data

Ontology terms mapped to Rusyn6 measures:

MA:0000358 liver
MA:0000368 kidney
MA:0002502 serum
MP:0000350 abnormal cell proliferation
MP:0000609 abnormal liver physiology
MP:0002136 abnormal kidney physiology
MP:0002707 abnormal kidney weight
MP:0004847 abnormal liver weight
MP:0005265 abnormal blood urea nitrogen level
MP:0008469 abnormal protein level
MP:0008872 abnormal physiological response to xenobiotic
MP:0008875 abnormal xenobiotic pharmacokinetics
MP:0011439 abnormal kidney cell proliferation
MP:0012696 abnormal trichloroethene metabolism
VT:0000609 liver physiology trait
VT:0002121 metabolism trait
VT:0002135 kidney morphology trait
VT:0002136 kidney physiology trait
VT:0002707 kidney mass
VT:0003402 liver mass
VT:0003893 hepatocyte proliferation trait
VT:0005265 blood urea nitrogen amount
VT:0010120 protein amount
VT:0010487 response to xenobiotic stimulus trait

Investigators	Ivan Rusyn Texas A&M University, College Station, TX
Participants	Yoo HS, Bradford BU, Kosyk O, Shymonyak S, Uehara T, Collins LB, Bodnar WM, Ball LM, Gold A
Contact	Ivan Rusyn IRusyn@cvm.tamu.edu Lab web site
Acknowledgements	Funding provided by NIH ES005948, ES010126; AstraZeneca
Project type	Phenotype strain survey data set
MPD identifiers	Rusyn6 MPD:512
Data changelog	No updates/corrections. Initial release date: 06/2014.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

A population-based approach was used to test the hypothesis that trichloroethylene (TCE) induced toxicity is associated with the formation of oxidative TCE metabolites. Liver, kidney and serum samples were tested. Major metabolizing enzymes responsible for oxidative biotransformation of TCE, as well as markers of liver and kidney cell proliferation and kidney damage were examined.

Procedures conducted:

• organ weights		Liver, kidney weights. Trichloroethylene-exposed vs. control.
• metabolic panel		Blood urea nitrogen (serum). Trichloroethylene-exposed vs. control.
• drug and metabolite quantification		Trichloroethylene metabolite in blood, liver, kidney after trichloroethylene exposure.
• RNA expression profiling		Trichloroethylene effect on mRNA gene expression in liver and kidney, relative to controls.
• histopathology		Kidney histopathology evaluation after trichloroethylene exposure, relative to controls.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Rusyn6:2	Rusyn6	drug and metabolite quantification	trichloroethylene	trichloroacetic acid (serum TCA) [nmol/mL] TCA_serum_tce 51202	inbred	7	m	6-7wks	N=3

Rusyn6:4	Rusyn6	drug and metabolite quantification	trichloroethylene	dichloroacetic acid (serum DCA) [nmol/mL] DCA_serum_tce 51204	inbred	7	m	6-7wks	N=3

Rusyn6:6	Rusyn6	drug and metabolite quantification	trichloroethylene	S-(1,2-dichlorovinyl) glutathione (serum DCVG) [pmol/mL] DCVG_serum_tce 51206	inbred	4	m	6-7wks	N=2

Rusyn6:8	Rusyn6	drug and metabolite quantification	trichloroethylene	S-(1,2-dichlorovinyl)-L-cysteine (serum DCVC) [pmol/mL] DCVC_serum_tce 51208	inbred	7	m	6-7wks	N=3

Rusyn6:9	Rusyn6	metabolic panel	trichloroethylene	blood urea nitrogen (serum BUN) [mg/dL] BUN 51209 control TCE	inbred	7	m	6-7wks	N=4

Rusyn6:11	Rusyn6	organ weights	trichloroethylene	liver weight as a percent of body weight [%] liver_adj 51211 control TCE	inbred	7	m	6-7wks	N=4

Rusyn6:14	Rusyn6	histopathology	trichloroethylene	BrdU-positive nuclei in liver (hepatocellular proliferation), relative to control group [%] BrdU_liver_tce 51214	inbred	7	m	6-7wks	N=3

Rusyn6:16	Rusyn6	drug and metabolite quantification	trichloroethylene	trichloroacetic acid (liver TCA) [nmol/g] TCA_liver_tce 51216	inbred	7	m	6-7wks	N=4

Rusyn6:18	Rusyn6	drug and metabolite quantification	trichloroethylene	dichloroacetic acid (liver DCA) [nmol/g] DCA_liver_tce 51218	inbred	7	m	6-7wks	N=4

Rusyn6:20	Rusyn6	drug and metabolite quantification	trichloroethylene	trichloroethanol (liver TCOH) [nmol/g] TCOH_liver_tce 51220	inbred	7	m	6-7wks	N=4

Rusyn6:22	Rusyn6	drug and metabolite quantification	trichloroethylene	S-(1,2-dichlorovinyl) glutathione (liver DCVG) [pmol/g] DCVG_liver_tce 51222	inbred	7	m	6-7wks	N=4

Rusyn6:26	Rusyn6	RNA expression profiling	trichloroethylene	Acox1, mRNA in liver, relative to control group [ratio] Acox1_liver_tce 51226	inbred	7	m	6-7wks	N=3

Rusyn6:28	Rusyn6	RNA expression profiling	trichloroethylene	Cyp4a10, mRNA in liver, relative to control group [ratio] Cyp4a10_liver_tce 51228	inbred	7	m	6-7wks	N=3

Rusyn6:30	Rusyn6	RNA expression profiling	trichloroethylene	Ppara, mRNA in liver, relative to control group [ratio] Ppara_liver_tce 51230	inbred	7	m	6-7wks	N=3

Rusyn6:51	Rusyn6	organ weights	trichloroethylene	kidney weight as a percent of body weight [%] kidney_adj 51251 control TCE	inbred	7	m	6-7wks	N=4

Rusyn6:54	Rusyn6	histopathology	trichloroethylene	BrdU-positive nuclei in kidney (proximal tubule cell proliferation), relative to control group [%] BrdU_kidney_tce 51254	inbred	7	m	6-7wks	N=3

Rusyn6:55	Rusyn6	histopathology	trichloroethylene	kidney injury molecule 1 (KIM-1) positive proximal tubules [%] KIM1_kidney 51255 control TCE	inbred	7	m	6-7wks	N=4

Rusyn6:58	Rusyn6	drug and metabolite quantification	trichloroethylene	trichloroacetic acid (kidney TCA) [nmol/g] TCA_kidney_tce 51258	inbred	7	m	6-7wks	N=4

Rusyn6:60	Rusyn6	drug and metabolite quantification	trichloroethylene	dichloroacetic acid (kidney DCA) [nmol/g] DCA_kidney_tce 51260	inbred	6	m	6-7wks	N=3

Rusyn6:62	Rusyn6	drug and metabolite quantification	trichloroethylene	trichloroethanol (kidney TCOH) [nmol/g] TCOH_kidney_tce 51262	inbred	7	m	6-7wks	N=4

Rusyn6:64	Rusyn6	drug and metabolite quantification	trichloroethylene	S-(1,2-dichlorovinyl) glutathione (kidney DCVG) [pmol/g] DCVG_kidney_tce 51264	inbred	6	m	6-7wks	N=2

Rusyn6:66	Rusyn6	drug and metabolite quantification	trichloroethylene	S-(1,2-dichlorovinyl)-L-cysteine (kidney DCVC) [pmol/g] DCVC_kidney_tce 51266	inbred	6	m	6-7wks	N=2

Rusyn6:68	Rusyn6	RNA expression profiling	trichloroethylene	Acox1, mRNA in kidney, relative to control group [ratio] Acox1_kidney_tce 51268	inbred	7	m	6-7wks	N=3

Rusyn6:70	Rusyn6	RNA expression profiling	trichloroethylene	Cyp4a10, mRNA in kidney, relative to control group [ratio] Cyp4a10_kidney_tce 51270	inbred	7	m	6-7wks	N=3

Rusyn6:72	Rusyn6	RNA expression profiling	trichloroethylene	Ppara, mRNA in kidney, relative to control group [ratio] Ppara_kidney_tce 51272	inbred	7	m	6-7wks	N=3

Rusyn6: Trichloroethylene metabolism and tissue-specific toxicity in males of 7 inbred strains of mice (2014)

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