MPD: Data set: Shorter5

Investigators	John R Shorter University of North Carolina, Chapel Hill, NC Deborah A O'Brien University of North Carolina, Chapel Hill, NC Fernando Pardo-Manuel de Villena University of North Carolina, Chapel Hill, NC
Participants	Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L
Contact	Fernando Pardo-Manuel de Villena fernando@med.unc.edu Lab web site
Part of a series:	• Organ weights, sperm count and morphology (this project) Shorter5 • Sperm motility see Shorter7
Acknowledgements	Funding provided by NIH HD065024; Eunice Kennedy Shriver National Institute of Child Health and Human Development. We would like to thank Lisa Branstetter, T. Justin Gooch, Stephanie Hansen, Ginger Shaw, Jennifer Shockley and Jason Spence for mouse work.
Project type	Phenotype strain survey data set
MPD identifiers	Shorter5 MPD:655
Data changelog	No updates/corrections. Initial release date: 06/2020.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Male reproductive parameters were assessed. This project contains data for organ weights and sperm count, motility, and morphology while Shorter7 contains Computer Assisted Sperm Analysis (CASA) data.

Procedures conducted:

• body weight
• organ weights		Male reproductive organ weights. Testes, seminal vesicles, epididymis + vas deferens.
• sperm count		Sperm count. Hemocytometer.
• microscopy		Sperm motility
• histopathology		Male reproductive histopathology. Percent normal, abnormal sperm.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Shorter5:1	Shorter5	body weight	(none)	body weight [g] bw 110118	CC RIX	28	m	7-44wks	N=4

Shorter5:2	Shorter5	organ weights	(none)	testis weight [g] testis 110143 left right	CC RIX	28	m	7-44wks	N=4

Shorter5:4	Shorter5	organ weights	(none)	testis weight mean [g] testis_mean 110121	CC RIX	28	m	7-44wks	N=4

Shorter5:5	Shorter5	organ weights	(none)	testis weight as percentage of body weight [%] testis_pct_bw 110122	CC RIX	28	m	7-44wks	N=4

Shorter5:6	Shorter5	organ weights	(none)	epididymis plus vas deferens weight [g] epidid_vd 110145 left right	CC RIX	28	m	7-44wks	N=4

Shorter5:8	Shorter5	organ weights	(none)	epididymis plus vas deferens weight mean [g] epidid_vd_mean 110125	CC RIX	28	m	7-44wks	N=4

Shorter5:9	Shorter5	organ weights	(none)	epididymis plus vas deferens weight as percentage of body weight [%] epidid_vd_pct_bw 110126	CC RIX	28	m	7-44wks	N=4

Shorter5:10	Shorter5	organ weights	(none)	seminal vesicles weight total [g] semin_vesi_tot 110127	CC RIX	28	m	7-44wks	N=4

Shorter5:11	Shorter5	organ weights	(none)	seminal vesicles weight as percentage of body weight [%] semin_vesi_pct_bw 110128	CC RIX	28	m	7-44wks	N=4

Shorter5:12	Shorter5	sperm count	(none)	sperm count per mouse (x 10⁶) [n] sperm_count 110129	CC RIX	28	m	7-44wks	N=4

Shorter5:13	Shorter5	sperm count	(none)	sperm count per mg testis weight (x 10³) [n/mg] sperm_adj 110130	CC RIX	28	m	7-44wks	N=4

Shorter5:14	Shorter5	microscopy	(none)	percentage of sperm that are motile [%] motile 110147 10 min 90 min	CC RIX	28	m	7-44wks	N=4

Shorter5:16	Shorter5	microscopy	(none)	percentage of motile sperm that are 'vigorous' [%] vigorous 110149 10 min 90 min	CC RIX	28	m	7-44wks	N=4

Shorter5:18	Shorter5	microscopy	(none)	percentage of motile sperm that are 'hyperactivated' [%] hyper 110151 10 min 90 min	CC RIX	28	m	7-44wks	N=4

Shorter5:20	Shorter5	microscopy	(none)	sperm mean curvilinear velocity [µm/s] VCL 110153 10 min 90 min	CC RIX	28	m	7-44wks	N=4

Shorter5:22	Shorter5	histopathology	(none)	percentage of sperm with normal morphology [%] normal_sperm 110139	CC RIX	28	m	7-44wks	N=4

Shorter5:23	Shorter5	histopathology	(none)	percentage of sperm with abnormal head shape [%] abnormal_head 110140	CC RIX	28	m	7-44wks	N=4

Shorter5:24	Shorter5	histopathology	(none)	percentage of sperm with abnormal tail bending [%] abnormal_tail 110141	CC RIX	28	m	7-44wks	N=4

Shorter5:25	Shorter5	histopathology	(none)	percentage of sperm with broken tails [%] broken_tail 110142	CC RIX	28	m	7-44wks	N=4

Shorter5: Male reproduction in 28 F1 hybrids of Collaborative Cross strains of mice (RIX): Organ weights and sperm count, motility, and morphology (2020)

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