MPD: Data set: Loos2

Loos M, Koopmans B, Aarts E, Maroteaux G, van der Sluis S; Neuro-BSIK Mouse Phenomics Consortium, Verhage M, Smit AB. Sheltering behavior and locomotor activity in 11 genetically diverse common inbred mouse strains using home-cage monitoring. PLoS One. 2014 Sep 29;9(9):e108563. doi: 10.1371/journal.pone.0108563. eCollection 2014. PubMed 25264768 FullText

Loos2 downloads
• Download Loos2 project data set animal data, as uploaded
• Download Loos2 animal data matrix with factor-related expansion applied as necessary
• Download Loos2 strain means, SD, N, etc. one row per strain/sex/measure
• Loos2 supplementary data

Ontology terms mapped to Loos2 measures:

MP:0001392 abnormal locomotor behavior
MP:0014039 abnormal sheltering behavior
MP:0020467 abnormal circadian behavior
VT:0001392 locomotor behavior trait
VT:0001502 behavioral circadian rhythm trait
VT:0010708 sheltering behavior trait

Investigators	Maarten Loos Sylics, Amsterdam NETHERLANDS Bastijn Koopmans Sylics, Amsterdam NETHERLANDS
Participants	Aarts E, Maroteaux G, van der Sluis S, Verhage M, Smit AB, Neuro-BSIK Mouse Phenomics Consortium
Contact	Maarten Loos maarten.loos@sylics.com Lab web site
Acknowledgements	Funding provided by Agentschap NL (NeuroBSIK Mouse Phenomics Consortium, BSIK03053); NeuroBasic PharmaPhenomics Consortium (LSH framework FES0908); Netherlands Organization for Scientific Research (NWO/MaGW: VIDI-452-12-014)
Project type	Phenotype strain survey data set
MPD identifiers	Loos2 MPD:540
Data changelog	No updates/corrections. Initial release date: 04/2016.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

This study aimed to determine the aspects of spontaneous mouse homecage behavior that are most influenced by genetic variation. Behavior was recorded for three consecutive days in a home-cage system by 24/7 overhead video tracking for each animal individually. Continuous video-tracking observations of sheltering behavior and locomotor activity were segmented into distinguishable behavioral elements, yielding a set of 115 behavioral parameters which demonstrated high dimensionality. Sixty-seven of those measurements have been accessioned here. Within-strain variation in behavior was also assessed. Selected measurements were log10 transformed by the investigators.

Procedures conducted:

• home cage monitoring

Locomotor activity, sheltering, habituation. 3 day home-cage assessment.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Loos2:1	Loos2	home cage monitoring	(none)	cumulative number of activity bouts, over 3 days of testing, log10 transformed [log(n)] activity_num 54001 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:3	Loos2	home cage monitoring	(none)	cumulative duration of activity during dark phase, over 3 days of testing [s] activity_dur_DK 54003	inbred w/CC7	11	m	8-12wks	N=40

Loos2:4	Loos2	home cage monitoring	(none)	cumulative duration of activity during light phase, over 3 days of testing, log10 transformed [log(s)] activity_dur_LT 54004	inbred w/CC7	11	m	8-12wks	N=40

Loos2:5	Loos2	home cage monitoring	(none)	mean duration per activity bout, over 3 days of testing, log10 transformed [log(s)] activity_mean_dur 54005 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:7	Loos2	home cage monitoring	(none)	habituation ratio describing change in cumulative activity duration, over 3 days of testing, log10 transformed [log(ratio)] activity_dur_hab 54007 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:11	Loos2	home cage monitoring	(none)	dark-light index describing difference in cumulative activity duration between light and dark phase, over 3 days of testing [ratio] activity_dur_DKLT_index 54011	inbred w/CC7	11	m	8-12wks	N=40

Loos2:12	Loos2	home cage monitoring	(none)	cumulative duration in spout zone, over 3 days of testing, log10 transformed [log(s)] spout_dur 54012	inbred w/CC7	11	m	8-12wks	N=40

Loos2:14	Loos2	home cage monitoring	(none)	cumulative duration in feeding zone during dark phase, over 3 days of testing, log10 transformed [log(s)] feeding_dur_DK 54014	inbred w/CC7	11	m	8-12wks	N=40

Loos2:15	Loos2	home cage monitoring	(none)	feeding zone, duration [s] feeding_dur_LT 54015	inbred w/CC7	11	m	8-12wks	N=40

Loos2:16	Loos2	home cage monitoring	(none)	cumulative number of visits to OnShelter zone, over 3 days of testing, log10 transformed [log(n)] onshelter_num 54016 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:18	Loos2	home cage monitoring	(none)	cumulative duration in OnShelter zone, over 3 days of testing, log10 transformed [log(s)] onshelter_dur 54018 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:22	Loos2	home cage monitoring	(none)	change in activity proportion: anticipation of phase change, over 3 days of testing activity_anticipation 54022 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:24	Loos2	home cage monitoring	(none)	change in activity proportion: response to phase onset, over 3 days of testing activity_response 54024 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:26	Loos2	home cage monitoring	(none)	change in Spout zone duration: anticipation of phase change, over 3 days of testing spout_anticipation 54026 dark light	inbred w/CC7	11	m	8-12wks	N=35

Loos2:28	Loos2	home cage monitoring	(none)	change in Spout zone duration: response to phase onset, over 3 days of testing spout_response 54028 dark light	inbred w/CC7	11	m	8-12wks	N=36

Loos2:32	Loos2	home cage monitoring	(none)	change in Feeding zone duration: anticipation of phase change, over 3 days of testing feeding_anticipation 54032 dark light	inbred w/CC7	11	m	8-12wks	N=35

Loos2:34	Loos2	home cage monitoring	(none)	change in Feeding zone duration: response to phase onset, over 3 days of testing feeding_response 54034 dark light	inbred w/CC7	11	m	8-12wks	N=36

Loos2:36	Loos2	home cage monitoring	(none)	change in OnShelter zone duration: anticipation of phase change, over 3 days of testing onshelter_anticipation 54036 dark light	inbred w/CC7	11	m	8-12wks	N=35

Loos2:38	Loos2	home cage monitoring	(none)	change in OnShelter zone duration: response to phase onset, over 3 days of testing onshelter_response 54038 dark light	inbred w/CC7	11	m	8-12wks	N=36

Loos2:42	Loos2	home cage monitoring	(none)	cumulative long movement number, over 3 days of testing, log10 transformed [log(n)] long_move_num 54042 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:44	Loos2	home cage monitoring	(none)	cumulative long movement distance, over 3 days of testing, log10 transformed [log(cm)] long_move_dist 54044 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:46	Loos2	home cage monitoring	(none)	average velocity of 95th percentile fastest long movement segments, over 3 days of testing, log10 transformed [log(cm/s)] long_move_max_vel 54046	inbred w/CC7	11	m	8-12wks	N=40

Loos2:47	Loos2	home cage monitoring	(none)	cut-off value to separate short and long movements, over 3 days of testing, log10 transformed [log(cm)] long_move_threshold 54047	inbred w/CC7	11	m	8-12wks	N=40

Loos2:48	Loos2	home cage monitoring	(none)	cumulative short movement number, over 3 days of testing, log10 transformed [log(n)] short_move_num 54048 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:52	Loos2	home cage monitoring	(none)	cumulative short movement distance, over 3 days of testing, log10 transformed [log(cm)] short_move_dist 54052 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:54	Loos2	home cage monitoring	(none)	cumulative number of long arrests, over 3 days of testing, log10 transformed [log(n)] long_arrest_num 54054 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:56	Loos2	home cage monitoring	(none)	cumulative duration of long arrests during dark phase, over 3 days of testing, log10 transformed [log(s)] long_arrest_dur_DK 54056	inbred w/CC7	11	m	8-12wks	N=40

Loos2:57	Loos2	home cage monitoring	(none)	long arrest, duration [s] long_arrest_dur_LT 54057	inbred w/CC7	11	m	8-12wks	N=40

Loos2:58	Loos2	home cage monitoring	(none)	mean duration per long arrest, over 3 days of testing, log10 transformed [log(s)] long_arrest_dur_mean 54058 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:62	Loos2	home cage monitoring	(none)	cut-off value to separate short and long arrests, over 3 days of testing, log10 transformed [log(s)] long_arrest_threshold 54062	inbred w/CC7	11	m	8-12wks	N=40

Loos2:63	Loos2	home cage monitoring	(none)	cumulative number of short arrests, over 3 days of testing, log10 transformed [log(n)] short_arrest_num 54063 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:65	Loos2	home cage monitoring	(none)	cumulative duration of short arrests during dark phase, over 3 days of testing [s] short_arrest_dur_DK 54065	inbred w/CC7	11	m	8-12wks	N=40

Loos2:66	Loos2	home cage monitoring	(none)	short arrest, duration [log(s)] short_arrest_dur_LT 54066	inbred w/CC7	11	m	8-12wks	N=40

Loos2:67	Loos2	home cage monitoring	(none)	mean duration per short arrest, over 3 days of testing, log10 transformed [log(s)] short_arrest_dur_mean 54067 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:71	Loos2	home cage monitoring	(none)	cumulative number of long shelter visits (sheltering), over 3 days of testing, log10 transformed [log(n)] long_shelter_num 54071 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:73	Loos2	home cage monitoring	(none)	cumulative duration of long shelter visits (sheltering), over 3 days of testing [s] long_shelter_dur 54073 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:75	Loos2	home cage monitoring	(none)	fraction of shelter visits (sheltering) with duration longer than long shelter visit threshold, over 3 days of testing, log10 transformed [log(ratio)] long_shelter_fraction 54075	inbred w/CC7	11	m	8-12wks	N=40

Loos2:76	Loos2	home cage monitoring	(none)	cut-off value to separate intermediate and long shelter visits (sheltering), over 3 days of testing [s] long_shelter_threshold 54076	inbred w/CC7	11	m	8-12wks	N=40

Loos2:77	Loos2	home cage monitoring	(none)	cumulative number of short shelter visits (sheltering), over 3 days of testing, log10 transformed [log(n)] short_shelter_num 54077 dark light	inbred w/CC7	11	m	8-12wks	N=40

Loos2:81	Loos2	home cage monitoring	(none)	cumulative duration of short shelter visits (sheltering) during dark phase, over 3 days of testing [s] short_shelter_dur_DK 54081	inbred w/CC7	11	m	8-12wks	N=40

Loos2:82	Loos2	home cage monitoring	(none)	short shelter visit, duration [log(s)] short_shelter_dur_LT 54082	inbred w/CC7	11	m	8-12wks	N=40

Loos2:83	Loos2	home cage monitoring	(none)	cut-off value to separate short and intermediate shelter visits (sheltering), over 3 days of testing [log(s)] short_shelter_threshold 54083	inbred w/CC7	11	m	8-12wks	N=40

Loos2:90	Loos2	home cage monitoring	(none)	mean within-strain variation in 22 principal components representing 115 spontaneous behavioral parameters within_strain_var 54401	inbred w/CC7	8	m	8-12wks

Loos2: Sheltering behavior and locomotor activity in males of 11 inbred strains of mice and within-strain variation of 8 of those strains (2016)