MPD: Data set: Graham1

Graham JB, Thomas S, Swarts J, McMillan AA, Ferris MT, Suthar MS, Treuting PM, Ireton R, Gale M Jr, Lund JM. Genetic diversity in the collaborative cross model recapitulates human West Nile virus disease outcomes. MBio. 2015 May 5;6(3):e00493-15. doi: 10.1128/mBio.00493-15. PubMed 25944860

Graham1 downloads
• Download Graham1 project data set animal data, as uploaded
• Download Graham1 animal data matrix with factor-related expansion applied as necessary
• Download Graham1 strain means, SD, N, etc. one row per strain/sex/measure
• Graham1 supplementary data

Ontology terms mapped to Graham1 measures:

MA:0000141 spleen
MA:0000168 brain
MA:0000368 kidney
MA:0002502 serum
MP:0001259 abnormal body weight
MP:0008073 abnormal CD4-positive, alpha beta T cell number
MP:0008077 abnormal CD8-positive, alpha-beta T cell number
MP:0008550 abnormal circulating interferon-beta level
MP:0012773 abnormal CD4-positive, CD25-positive, alpha-beta regulatory T cell number
MP:0020180 abnormal IgM level
MP:0020185 altered susceptibility to viral infection
VT:0001259 body mass
VT:0008073 CD4-positive T cell quantity
VT:0008077 CD8-positive T cell quantity
VT:0008397 CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology trait
VT:0008550 blood interferon-beta amount
VT:0010439 response to viral infection trait
VT:0010481 blood immunoglobulin M amount

Investigators	Jessica B Graham Fred Hutchinson Cancer Research Center, Seattle, WA Sunil Thomas University of Washington, Seattle, WA Michael Gale Jr. University of Washington, Seattle, WA Jennifer M Lund Fred Hutchinson Cancer Research Center, Seattle, WA
Participants	Swarts J, McMillan AA, Ferris MT, Suthar MS, Treuting PM, Ireton R
Contact	Jessica B Graham jgraham@fredhutch.org Lab web site
Acknowledgements	Funding provided by NIH AI100625, AI04002, AI083019
Project type	Phenotype strain survey data set
MPD identifiers	Graham1 MPD:633
Data changelog	No updates/corrections. Initial release date: 11/2018.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Experimental groups in this study:
• None • None • None • None • None • None • None • None • None

Procedures conducted:

• body weight
• health assessment		Clinical score.
• immune cell quantification		T cell populations in brain and spleen.
• RNA expression profiling		Viral load and expression of Ifnb1 and Ifit1 in brain, kidney and spleen.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Graham1:1	Graham1	body weight	West Nile virus	body weight, post infection [g] bw 63301 day 0 day 3 day 6 day 8 day 9 day 10 day 11 day 12 day 13 day 14 (4 more)...	CC RIX	5	m	8-10wks	N=12

Graham1:15	Graham1	health assessment	West Nile virus	clinical score (0=healthy, 1=lethargic, 2=altered gait, 3=no movement, 4=moribund), post infection [score] clin_score 63315 day 0 day 3 day 6 day 8 day 9 day 10 day 11 day 12 day 13 day 14 (4 more)...	CC RIX	5	m	8-10wks	N=12

Graham1:29	Graham1	immune cell quantification	West Nile virus	percentage of CD3+ T cells that are CD4+ in brain, post infection [%] CD4_brain 63329 day 7 day 12 day 21 day 28	CC RIX	5	m	8-10wks	N=3

Graham1:33	Graham1	immune cell quantification	West Nile virus	percentage of CD3+ T cells that are CD4+ in spleen, post infection [%] CD4_spleen 63333 day 7 day 12 day 21 day 28	CC RIX	2	m	8-10wks	N=3

Graham1:37	Graham1	immune cell quantification	West Nile virus	percentage of CD4+ T cells that are Foxp3+ in the brain, post infection [%] Treg_brain 63337 day 7 day 12 day 21 day 28	CC RIX	5	m	8-10wks	N=3

Graham1:41	Graham1	immune cell quantification	West Nile virus	percentage of CD4+ T cells that are Foxp3+ in the spleen, post infection [%] Treg_spleen 63341 day 7 day 12 day 21 day 28	CC RIX	2	m	8-10wks	N=3

Graham1:45	Graham1	immune cell quantification	West Nile virus	percentage of CD3+ T cells that are CD8+ in brain, post infection [%] CD8_brain 63345 day 7 day 12 day 21 day 28	CC RIX	5	m	8-10wks	N=3

Graham1:49	Graham1	immune cell quantification	West Nile virus	percentage of CD3+ T cells that are CD8+ in spleen, post infection [%] CD8_spleen 63349 day 7 day 12 day 21 day 28	CC RIX	2	m	8-10wks	N=3

Graham1:53	Graham1	immune cell quantification	West Nile virus	percentage of CD3+ T cells that are NS4b+ in brain, post infection [%] NS4b_brain 63353 day 7 day 12 day 21 day 28	CC RIX	5	m	8-10wks	N=3

Graham1:57	Graham1	immune cell quantification	West Nile virus	percentage of CD3+ T cells that are NS4b+ in spleen, post infection [%] NS4b_spleen 63357 day 7 day 12 day 21 day 28	CC RIX	2	m	8-10wks	N=3

Graham1:61	Graham1	immunoglobulin quantification	West Nile virus	virus-specific IgM in serum, post infection [ng/mL] WNV_IgM 63361 day 7 day 12 day 21 day 28	CC RIX	5	m	8-10wks	N=3

Graham1:65	Graham1	RNA expression profiling	West Nile virus	virus in brain, fold change compared to matched control, post infection WNV_brain 63365 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:68	Graham1	RNA expression profiling	West Nile virus	virus in kidney, fold change compared to matched control, post infection WNV_kidney 63368 day 2 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:72	Graham1	RNA expression profiling	West Nile virus	virus in spleen, fold change compared to matched control, post infection WNV_spleen 63372 day 2 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:76	Graham1	RNA expression profiling	West Nile virus	Ifnb1 gene expression in brain, fold change compared to matched control, post infection Ifnb1_brain 63376 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:79	Graham1	RNA expression profiling	West Nile virus	Ifnb1 gene expression in kidney, fold change compared to matched control, post infection Ifnb1_kidney 63379 day 2 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:83	Graham1	RNA expression profiling	West Nile virus	Ifnb1 gene expression in spleen, fold change compared to matched control, post infection Ifnb1_spleen 63383 day 2 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:87	Graham1	RNA expression profiling	West Nile virus	Ifit1 gene expression in brain, fold change compared to matched control, post infection Ifit1_brain 63387 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:90	Graham1	RNA expression profiling	West Nile virus	Ifit1 gene expression in kidney, fold change compared to matched control, post infection Ifit1_kidney 63390 day 2 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

Graham1:94	Graham1	RNA expression profiling	West Nile virus	Ifit1 gene expression in spleen, fold change compared to matched control, post infection Ifit1_spleen 63394 day 2 day 4 day 7 day 12	CC RIX	5	m	8-10wks	N=1

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Graham1: West Nile virus infection in males of F1 hybrids (RIX) of Collaborative Cross strains of mice (2015)

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Graham1: West Nile virus infection in males of F1 hybrids (RIX) of Collaborative Cross strains of mice (2015)

Filter Table Results

An error occurred on this page.
These results could be incomplete or invalid. Staff have been notified.