Acclimation & testing periods
VendorTen inbred strains of mice of both genders were purchased from Jackson Laboratory (JAX®, Bar Harbor, ME). AKR males were not studied because only AKR females were available from the supplier. AgesMice were 11-12 wks of age on the day of sacrifice FeedPurina mouse chow 5053 containing 4.5% fat, 20.0% protein, and 54.8% carbohydrate (LabDiet) WaterAd libitum Housinggroup-housed 4-5 mice of the same sex per cage Room temperature24°C Beddingcorncob; aspen chips during GTT and ITT days Photoperiod12h/12h light/dark cycle (6:00 AM to 6:00 PM) Health statusAll animal procedures were performed in accordance with guidelines of Washington University's Animal Studies Committee and approved by the IACUC of Washington University.