Acclimation testing periods
VendorMale mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME), and shipped by air at 4–7 wks of age. AcclimationMice were acclimated to the vivarium for at least 1 wk prior to testing or surgery at 7–10 wks of age. Ages 6-18 wks Feedad libitum (Harlan Teklad Laboratory Diet) Waterad libitum HousingMice were housed with in groups of four or less Photoperiod12 light 12 dark cycle (lights on at 07:00 h) Temperature22 ±1 °C