GMC16 project protocol

Clinical blood chemistry for 8 inbred founder strains of the Collaborative Cross   (2020)

German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Rathkolb B, Wolf E, Lengger C, Kollmus H, Schughart K




German Mouse Clinic: Phenotyping Pipeline

Clinical blood chemistry: 16-17 and 20-21 wks of age

 

  • Project protocol - Contents

    Workflow and sampling

    Step
    Procedure
    		 Equipment
    Data collected
    1 Body weight measured Balance Body weight
    2 Mice anesthetized; retro-orbital bleed Lithium-heparin tubes -
    3 Samples processed and analyzed Clinical chemistry analyzer Clinical chemistry parameters

    Equipment and supplies

    • Balance: Ohaus (AV213C)
    • Lithium-heparin blood collection tubes
    • Vortex
    • Centrifuge
    • Clinical chemistry analyzer: Beckman-Coulter (AU480)

    Reagents and solutions

    • Isoflurane
    • Beckman-Coulter System reagents

    Procedure: Clinical chemistry

      1. Mice (non-fasting) are anesthetized via isoflurane inhalation.
      2. Blood is collected in the morning by retro-orbital puncture (~200 µL) in lithium heparin-coated tubes, and stored at room temperature for 1-2h maximum.
      3. Samples are centrifuged for 10 min at 5000 x g in a refrigerated centrifuge set at 8°C.
      4. Analysis is optimally done the day of blood collection (when not possible, samples are frozen at -20°C until analysis); all samples are run at room temperature.
      5. If necessary, samples are diluted 1:2 in deionized water.
      6. Samples must be free of fibrin clots (can remove with wooden applicator stick).
      7. Samples are run according to manufacturer's instructions.

    Data collected by investigator

    • Body weight
    • Plasma electrolytes
    • Plasma enzymes
    • Plasma glucose
    • Plasma proteins
    • Plasma urea
    • Plasma lipids
    • Plasma unsaturated iron binding capacity

    Supplementary data are available for this project. See GMC16 Downloads.