MPD: Data set: Chesler3

Chesler3: Multi-system assessment of 8 inbred founder strains and 360+ emerging lines (pre-CC) of the Collaborative Cross (2011)

Philip VM, Sokoloff G, Ackert-Bicknell CL, Striz M, Branstetter L, Beckmann MA, Spence JS, Jackson BL, Galloway LD, Barker P, Wymore AM, Hunsicker PR, Durtschi DC, Chesler EJ, et al. Genetic analysis in the Collaborative Cross breeding population. Genome Res. 2011 Aug;21(8):1223-38. Epub 2011 Jul 6. PubMed 21734011 FullText

Chesler3 downloads
• Download Chesler3 project data set animal data, as uploaded
• Download Chesler3 animal data matrix with factor-related expansion applied as necessary
• Download Chesler3 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Chesler3 measures:

MA:0000008 tail
MA:0000059 blood
MA:0000072 heart
MA:0000141 spleen
MA:0000168 brain
MA:0000326 reproductive system
MA:0000368 kidney
MA:0001359 femur
MA:0002501 plasma
MA:0002547 gonadal fat pad
MP:0000188 abnormal circulating glucose level
MP:0000217 abnormal leukocyte cell number
MP:0000226 abnormal mean corpuscular volume
MP:0000559 abnormal femur morphology
MP:0000717 abnormal lymphocyte cell number
MP:0000771 abnormal brain size
MP:0001256 abnormal body length
MP:0001259 abnormal body weight
MP:0001362 abnormal anxiety-related response
MP:0001392 abnormal locomotor behavior
MP:0001562 abnormal circulating calcium level
MP:0001586 abnormal erythrocyte cell number
MP:0001588 abnormal hemoglobin
MP:0001589 abnormal mean corpuscular hemoglobin
MP:0001761 abnormal urination pattern
MP:0001776 abnormal circulating sodium level
MP:0002061 abnormal aggression-related behavior
MP:0002161 abnormal fertility/fecundity
MP:0002586 abnormal platelet volume
MP:0002596 abnormal hematocrit
MP:0002602 abnormal eosinophil cell number
MP:0002668 abnormal circulating potassium level
MP:0002706 abnormal kidney size
MP:0002707 abnormal kidney weight
MP:0002733 abnormal thermal nociception
MP:0002734 abnormal mechanical nociception
MP:0003018 abnormal circulating chloride level
MP:0003860 abnormal carbon dioxide level
MP:0004214 abnormal long bone diaphysis morphology
MP:0004850 abnormal testis weight
MP:0004857 abnormal heart weight
MP:0004951 abnormal spleen weight
MP:0005265 abnormal blood urea nitrogen level
MP:0005328 abnormal circulating creatinine level
MP:0005335 abnormal gonadal fat pad morphology
MP:0005640 abnormal mean corpuscular hemoglobin concentration
MP:0006357 abnormal circulating mineral level
MP:0010066 abnormal red blood cell distribution width
MP:0011396 abnormal sleep behavior
MP:0011898 abnormal platelet cell number
MP:0011999 abnormal tail length
MP:0012441 abnormal monocyte cell number
MP:0013189 abnormal exploration in a new environment
MP:0020147 abnormal defecation amount
MP:0020201 abnormal granulocyte number
MP:0020467 abnormal circadian behavior
VT:0000068 blood mineral amount
VT:0000188 blood glucose amount
VT:0000217 leukocyte quantity
VT:0000223 monocyte quantity
VT:0000248 erythrocyte size trait
VT:0000334 granulocyte quantity
VT:0000689 spleen morphology trait
VT:0000717 lymphocyte quantity
VT:0001256 body length
VT:0001259 body mass
VT:0001392 locomotor behavior trait
VT:0001501 sleep behavior trait
VT:0001502 behavioral circadian rhythm trait
VT:0001562 blood calcium amount
VT:0001586 erythrocyte quantity
VT:0001588 blood hemoglobin amount
VT:0001776 blood sodium amount
VT:0001922 male fertility trait
VT:0001923 female fertility trait
VT:0001970 pain threshold
VT:0002161 female fecundity trait
VT:0002176 brain mass
VT:0002329 blood gas amount
VT:0002447 erythrocyte morphology trait
VT:0002602 eosinophil quantity
VT:0002668 blood potassium amount
VT:0002706 kidney size trait
VT:0002707 kidney mass
VT:0002733 thermal nociception trait
VT:0002734 mechanical nociception trait
VT:0002758 tail length
VT:0003018 blood chloride amount
VT:0003179 platelet quantity
VT:0003620 urine output
VT:0004152 blood iron amount
VT:0004951 spleen mass
VT:0005265 blood urea nitrogen amount
VT:0005328 blood creatinine amount
VT:0005335 gonadal fat pad morphology trait
VT:0005452 adipose amount
VT:0007028 heart mass
VT:0010014 femur circumference
VT:0010457 platelet size trait
VT:0010461 excretion behavior trait
VT:0010462 defecation behavior trait
VT:0010464 fertility trait
VT:0010471 exploratory behavior trait
VT:0010716 anxiety-related behavior trait
VT:0010726 wildness behavior trait
VT:0015014 aggression-related behavior trait
VT:1000189 offspring quantity
VT:1000644 testis mass

Investigators	Elissa J Chesler The Jackson Laboratory, Bar Harbor, ME Vivek M Philip The Jackson Laboratory, Bar Harbor, ME Brynn H Voy University of Tennessee, Knoxville, TN
Participants	Sokoloff G, Ackert-Bicknell C, Striz M, Churchill GA, Lariviere WR, Palmer AA, OHara BF
Contact	Elissa J Chesler elissa.chesler@jax.org Lab web site
Part of a series:	• F1s with progenitors see Chesler2 • pre-CC lines w/ progenitors (this project) Chesler3
Acknowledgements	Funding provided by NIH DA021198, MH079103, AG025707; FA9550-05-1-0464; US Department of Energy, University of Tennessee Health Science Center, Oak Ridge National Laboratory, The Jackson Laboratory, The Ellison Medical Foundation
Project type	Phenotype strain survey data set
MPD identifiers	Chesler3 MPD:435
Data changelog	2 updates/corrections. Initial release date: 12/2012.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

An assessment of emerging CC lines. Generations range from G2:F5 to G2:F9. Data for the 8 founder strains are also present.

Procedures conducted:

• metabolic panel		Clinical blood chemistry. 16h fast.
• complete blood count		Hematology.
• colony observation		Reproduction, fecundity.
• open field test		Locomotor activity, exploratory, anxiety-related behavior. 10 min test.
• light-dark box		Anxiety, avoidance behavior. 10 min test.
• behavior observation		Wildness scoring.
• nociception assay		Hot plate and pressure on tail.
• monitoring system		Sleep patterns. Dark period, total, peak activity. Baseline and after sleep deprivation.
• body size and weight		Body weight, tail length.
• fat pad weights		Gonadal fat pad weights.
• bone dimensions		Femur.
• organ weights		Heart, brain, kidney, spleen, gonadal fat pads, testicle weights.
• organ dimensions		Kidney length and volume.

Mice: CC pre w/par 395 strains ♀♂ age 7-15wks 1 experimental group

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Chesler3:1	Chesler3	metabolic panel	(none)	sodium (blood Na) [mmol/L] sodium 43501	CC pre w/par	190	both	7-15wks	N=1

Chesler3:2	Chesler3	metabolic panel	(none)	potassium (blood K) [mmol/L] potassium 43502	CC pre w/par	190	both	7-15wks	N=1

Chesler3:3	Chesler3	metabolic panel	(none)	chloride (blood Cl) [mmol/L] chloride 43503	CC pre w/par	190	both	7-15wks	N=1

Chesler3:4	Chesler3	metabolic panel	(none)	calcium (blood ionized Ca) [mmol/L] calcium 43504	CC pre w/par	190	both	7-15wks	N=1

Chesler3:5	Chesler3	metabolic panel	(none)	anion gap concentration (mEq/L) [mmol/L] anion_gap 43505	CC pre w/par	187	both	7-15wks	N=1

Chesler3:6	Chesler3	metabolic panel	(none)	carbon dioxide (blood CO₂) [mmol/L] carbon_dioxide 43506	CC pre w/par	189	both	7-15wks	N=1

Chesler3:7	Chesler3	metabolic panel	(none)	glucose (blood GLU, no fast) [mg/dL] GLU 43507	CC pre w/par	190	both	7-15wks	N=1

Chesler3:8	Chesler3	metabolic panel	(none)	glucose (blood GLU, 16h fast) [mg/dL] GLU_fast 43508	CC pre w/par	275	both	7-15wks	N=2

Chesler3:9	Chesler3	metabolic panel	(none)	blood urea nitrogen (BUN) [mg/dL] BUN 43509	CC pre w/par	190	both	7-15wks	N=1

Chesler3:10	Chesler3	metabolic panel	(none)	creatinine (blood) [mg/dL] creatinine 43510	CC pre w/par	189	both	7-15wks	N=1

Chesler3:13	Chesler3	complete blood count	(none)	white blood cell count (WBC; units per volume x 10³) [n/µL] WBC 43513	CC pre w/par	199	both	7-15wks	N=1

Chesler3:14	Chesler3	complete blood count	(none)	lymphocyte count (LYM; units per volume x 10³) [n/µL] LYM 43514	CC pre w/par	198	both	7-15wks	N=1

Chesler3:15	Chesler3	complete blood count	(none)	lymphocyte differential (LYM; percentage of viable WBC) [%] pct_LYM 43515	CC pre w/par	198	both	7-15wks	N=1

Chesler3:16	Chesler3	complete blood count	(none)	monocyte count (MONO; units per volume x 10³) [n/µL] MONO 43516	CC pre w/par	198	both	7-15wks	N=1

Chesler3:17	Chesler3	complete blood count	(none)	monocyte differential (MONO; percentage of viable WBC) [%] pct_MONO 43517	CC pre w/par	198	both	7-15wks	N=1

Chesler3:18	Chesler3	complete blood count	(none)	granulocyte count (GRA; units per volume x 10³) [n/µL] GRA 43518	CC pre w/par	198	both	7-15wks	N=1

Chesler3:19	Chesler3	complete blood count	(none)	granulocyte differential (GRA; percentage of total viable WBC) [%] pct_GRA 43519	CC pre w/par	198	both	7-15wks	N=1

Chesler3:20	Chesler3	complete blood count	(none)	eosinophil differential (EOS; percentage of viable WBC) [%] pct_EOS 43520	CC pre w/par	198	both	7-15wks	N=1

Chesler3:21	Chesler3	complete blood count	(none)	red blood cell count (RBC; units per volume x 10⁶) [n/µL] RBC 43521	CC pre w/par	200	both	7-15wks	N=1

Chesler3:22	Chesler3	complete blood count	(none)	mean RBC corpuscular volume (MCV) [fL] MCV 43522	CC pre w/par	200	both	7-15wks	N=1

Chesler3:23	Chesler3	complete blood count	(none)	RBC corpuscular distribution width (RDW) [%] RDW 43523	CC pre w/par	200	both	7-15wks	N=1

Chesler3:24	Chesler3	complete blood count	(none)	mean RBC corpuscular hemoglobin (MCH) [pg] MCH 43524	CC pre w/par	200	both	7-15wks	N=1

Chesler3:25	Chesler3	complete blood count	(none)	mean RBC corpuscular hemoglobin concentration (MCHC) [g/dL] MCHC 43525	CC pre w/par	200	both	7-15wks	N=1

Chesler3:26	Chesler3	complete blood count	(none)	hemoglobin (HGB) [g/dL] hemoglobin 43526	CC pre w/par	200	both	7-15wks	N=1

Chesler3:27	Chesler3	complete blood count	(none)	hematocrit (HCT) [%] hematocrit 43527	CC pre w/par	200	both	7-15wks	N=1

Chesler3:28	Chesler3	complete blood count	(none)	platelet count (PLT; units per volume x 10³) [n/µL] platelets 43528	CC pre w/par	200	both	7-15wks	N=1

Chesler3:29	Chesler3	complete blood count	(none)	mean platelet volume (MPV) [fL] MPV 43529	CC pre w/par	200	both	7-15wks	N=1

Chesler3:40	Chesler3	organ weights	(none)	testicular weight [g] testes_wt 43540	CC pre w/par	54	both	4wks	N=1

Chesler3:41	Chesler3	colony observation	(none)	number of mice born in first litter [n] litter_size 43541	CC pre w/par	312	both	4wks	N=2

Chesler3:42	Chesler3	colony observation	(none)	parental age at first litter [d] age_first_litter 43542	CC pre w/par	312	both	4wks	N=2

Chesler3:43	Chesler3	colony observation	(none)	time from mate to first litter [d] duration_to_litter 43543	CC pre w/par	312	both	4wks	N=2

Chesler3:51	Chesler3	open field test	(none)	distance traveled during first 3 min, 10 min test [cm] distance3_OFT 43551	CC pre w/par	239	both	7-15wks	N=1

Chesler3:52	Chesler3	open field test	(none)	distance traveled, 10 min test [cm] distance10_OFT 43552	CC pre w/par	239	both	7-15wks	N=1

Chesler3:53	Chesler3	open field test	(none)	number of transitions between center and periphery, 10 min test [n] transitions_OFT 43553	CC pre w/par	235	both	7-15wks	N=1

Chesler3:54	Chesler3	open field test	(none)	time spent in center during first minute, 10 min test [s] center1_OFT 43554	CC pre w/par	239	both	7-15wks	N=1

Chesler3:55	Chesler3	open field test	(none)	time spent in center, 10 min test [s] center10_OFT 43555	CC pre w/par	239	both	7-15wks	N=1

Chesler3:56	Chesler3	open field test	(none)	integrated distance from absolute center, 10 min test [cm] distance_center_OFT 43556	CC pre w/par	237	both	7-15wks	N=1

Chesler3:57	Chesler3	open field test	(none)	percentage of time in center, 10 min test [%] pct_center_OFT 43557	CC pre w/par	239	both	7-15wks	N=1

Chesler3:58	Chesler3	open field test	(none)	latency to reach periphery, 10 min test [s] latency_periphery_OFT 43558	CC pre w/par	235	both	7-15wks	N=1

Chesler3:59	Chesler3	open field test	(none)	time spent in periphery, 10 min test [s] duration_periphery_OFT 43559	CC pre w/par	239	both	7-15wks	N=1

Chesler3:60	Chesler3	open field test	(none)	time spent immobile during first minute, 10 min test [s] immobile_OFT 43560	CC pre w/par	237	both	7-15wks	N=1

Chesler3:61	Chesler3	open field test	(none)	number of fecal boli, 10 min test [n] fecal_boli_OFT 43561	CC pre w/par	232	both	7-15wks	N=1

Chesler3:62	Chesler3	open field test	(none)	number of urine puddles, 10 min test [n] urination_OFT 43562	CC pre w/par	232	both	7-15wks	N=1

Chesler3:63	Chesler3	light-dark box	(none)	time spent in light compartment, 10 min test [s] duration_light_LD 43563	CC pre w/par	194	both	7-15wks	N=1

Chesler3:64	Chesler3	light-dark box	(none)	distance traveled in light compartment, 10 min test [cm] distance_light_LD 43564	CC pre w/par	194	both	7-15wks	N=1

Chesler3:65	Chesler3	light-dark box	(none)	percentage of time in light compartment, 10 min test [%] pct_light_LD 43565	CC pre w/par	196	both	7-15wks	N=1

Chesler3:66	Chesler3	light-dark box	(none)	latency to enter dark compartment, 10 min test [s] latency_dark_LD 43566	CC pre w/par	199	both	7-15wks	N=1

Chesler3:67	Chesler3	light-dark box	(none)	number of transitions between light and dark compartments, 10 min test [n] transitions_LD 43567	CC pre w/par	196	both	7-15wks	N=1

Chesler3:68	Chesler3	light-dark box	(none)	number of fecal boli, 10 min test [n] fecal_boli_LD 43568	CC pre w/par	193	both	7-15wks	N=1

Chesler3:69	Chesler3	light-dark box	(none)	number of urine puddles, 10 min test [n] urination_LD 43569	CC pre w/par	193	both	7-15wks	N=1

Chesler3:70	Chesler3	behavior observation	(none)	response to capture [score] capture_score 43570	CC pre w/par	350	both	7-15wks	N=2

Chesler3:71	Chesler3	behavior observation	(none)	response to holding [score] hold_score 43571	CC pre w/par	350	both	7-15wks	N=2

Chesler3:72	Chesler3	behavior observation	(none)	capture and hold scores combined [score] wildness_score 43572	CC pre w/par	350	both	7-15wks	N=2

Chesler3:73	Chesler3	nociception assay	(none)	latency of nociceptive response to pressure on the tail, 60 s test [s] tail_clip 43573	CC pre w/par	330	both	7-15wks	N=2

Chesler3:74	Chesler3	nociception assay	(none)	latency of nociceptive response to hot plate (54°C), 30 s test [s] hot_plate 43574	CC pre w/par	329	both	7-15wks	N=2

Chesler3:81	Chesler3	monitoring system	(none)	percentage of time sleeping, baseline [%] pct_sleep 43581 total dark period	CC pre w/par	301	both	7-15wks	N=2

Chesler3:83	Chesler3	monitoring system	(none)	percentage of time sleeping, after sleep deprivation [%] deprivation 43583 total dark period peak activity	CC pre w/par	301	both	7-15wks	N=2

Chesler3:86	Chesler3	body size and weight	(none)	body weight [g] body_weight 43586	CC pre w/par	362	both	7-15wks	N=3

Chesler3:87	Chesler3	body size and weight	(none)	body length [cm] body_length 43587	CC pre w/par	279	both	7-15wks	N=2

Chesler3:88	Chesler3	body size and weight	(none)	tail length [cm] tail_length 43588	CC pre w/par	352	both	7-15wks	N=2

Chesler3:89	Chesler3	fat pad weights	(none)	gonadal fat pad weight [g] gonad_fat_wt 43589	CC pre w/par	350	both	7-15wks	N=2

Chesler3:90	Chesler3	bone dimensions	(none)	periosteal (outer) circumference, femoral mid-diaphysis [mm] bone_circumference 43590	CC pre w/par	235	both	7-15wks	N=1

Chesler3:91	Chesler3	organ weights	(none)	brain weight [g] brain_wt 43591	CC pre w/par	181	both	7-15wks	N=1

Chesler3:92	Chesler3	organ weights	(none)	heart weight [g] heart_wt 43592	CC pre w/par	352	both	7-15wks	N=2

Chesler3:93	Chesler3	organ dimensions	(none)	kidney length [cm] kidney_length 43593 left right	CC pre w/par	124	both	7-15wks	N=1

Chesler3:95	Chesler3	organ dimensions	(none)	kidney volume [cm³] kidney_vol 43595	CC pre w/par	77	both	7-15wks	N=1

Chesler3:96	Chesler3	organ weights	(none)	kidney weight [g] kidney_wt 43596 left right both	CC pre w/par	351	both	7-15wks	N=2

Chesler3:99	Chesler3	organ weights	(none)	spleen weight [g] spleen_wt 43599	CC pre w/par	263	both	7-15wks	N=2

Chesler3: Multi-system assessment of 8 inbred founder strains and 360+ emerging lines (pre-CC) of the Collaborative Cross (2011)

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