Project measure / variable:   GMC16   iron_21

ID, description, units MPD:56515   iron_21   iron (plasma Fe)   [µmol/L]  at age 20-21wks  
Data set, strains GMC16   inbred w/CC8   8 strains     sex: both     age: 20-21wks
Procedure metabolic panel
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  STRAIN COMPARISON PLOT
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GMC16 - iron (plasma Fe) at age 20-21wks



  MEASURE SUMMARY
Measure Summary FemaleMale
Number of strains tested8 strains8 strains
Mean of the strain means31.9   µmol/L 30.0   µmol/L
Median of the strain means31.9   µmol/L 31.9   µmol/L
SD of the strain means± 5.47 ± 6.12
Coefficient of variation (CV)0.172 0.204
Min–max range of strain means24.6   –   38.9   µmol/L 18.1   –   37.1   µmol/L
Mean sample size per strain10.1   mice 11.5   mice


  ANOVA, Q-Q NORMALITY ASSESSMENT
ANOVA summary      
FactorDFSum of squaresMean sum of squaresF valuep value (Pr>F)
sex 1 178.0431 178.0431 9.2156 0.0028
strain 7 4652.5286 664.6469 34.4023 < 0.0001
sex:strain 7 545.8142 77.9735 4.0359 0.0004
Residuals 157 3033.2167 19.3199


Q-Q normality assessment based on residuals

  


  STRAIN MEANS (UNADJUSTED)
  
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Strain Sex Mean SD N mice SEM CV Min, Max Z score
129S1/SvImJ f 32.6 3.32   10 1.05 0.102 26.8, 36.7 0.13
129S1/SvImJ m 31.0 2.46   12 0.711 0.0795 25.8, 35.0 0.17
A/J f 38.9 3.44   10 1.09 0.0886 32.3, 43.2 1.28
A/J m 32.7 3.26   11 0.983 0.0997 27.9, 36.5 0.45
C57BL/6J f 24.6 4.72   10 1.49 0.192 17.9, 35.0 -1.33
C57BL/6J m 18.1 1.81   12 0.523 0.1 14.1, 21.6 -1.94
CAST/EiJ f 35.4 4.04   12 1.17 0.114 31.2, 45.5 0.64
CAST/EiJ m 34.4 3.58   11 1.08 0.104 29.6, 40.8 0.72
NOD/ShiLtJ f 29.7 9.1   9 3.03 0.306 17.6, 45.2 -0.4
NOD/ShiLtJ m 33.9 5.07   12 1.46 0.15 24.9, 40.8 0.64
NZO/HlLtJ f 24.7 2.25   10 0.713 0.0912 21.6, 27.3 -1.31
NZO/HlLtJ m 26.6 2.62   11 0.789 0.0984 21.8, 30.9 -0.55
PWK/PhJ f 31.2 7.39   10 2.34 0.237 21.3, 45.7 -0.13
PWK/PhJ m 26.0 5.31   11 1.6 0.205 14.5, 32.3 -0.65
WSB/EiJ f 38.0 2.77   10 0.877 0.073 34.4, 41.4 1.12
WSB/EiJ m 37.1 4.34   12 1.25 0.117 31.0, 45.0 1.16


  LEAST SQUARES MEANS (MODEL-ADJUSTED)
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ f 32.599 1.39 35.3444 29.8536
129S1/SvImJ m 30.9883 1.2689 33.4946 28.4821
A/J f 38.885 1.39 41.6304 36.1396
A/J m 32.6882 1.3253 35.3058 30.0705
C57BL/6J f 24.606 1.39 27.3514 21.8606
C57BL/6J m 18.1125 1.2689 20.6187 15.6063
CAST/EiJ f 35.4067 1.2689 37.9129 32.9004
CAST/EiJ m 34.4136 1.3253 37.0313 31.796
NOD/ShiLtJ f 29.7078 1.4651 32.6017 26.8138
NOD/ShiLtJ m 33.8908 1.2689 36.3971 31.3846
NZO/HlLtJ f 24.735 1.39 27.4804 21.9896
NZO/HlLtJ m 26.6064 1.3253 29.224 23.9887
PWK/PhJ f 31.203 1.39 33.9484 28.4576
PWK/PhJ m 25.96 1.3253 28.5777 23.3423
WSB/EiJ f 37.992 1.39 40.7374 35.2466
WSB/EiJ m 37.1017 1.2689 39.6079 34.5954


  LEAST SQUARES MEANS (MODEL-ADJUSTED), SEXES COMBINED
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ both 31.7937 0.941 33.6523 29.935
A/J both 35.7866 0.9603 37.6833 33.8899
C57BL/6J both 21.3592 0.941 23.2179 19.5006
CAST/EiJ both 34.9102 0.9174 36.7221 33.0982
NOD/ShiLtJ both 31.7993 0.9691 33.7135 29.8851
NZO/HlLtJ both 25.6707 0.9603 27.5674 23.774
PWK/PhJ both 28.5815 0.9603 30.4782 26.6848
WSB/EiJ both 37.5468 0.941 39.4055 35.6882




  GWAS USING LINEAR MIXED MODELS