MPD: Xing1: animal documentation

Xing1 animal documentation

Aging study: Electrocardiogram for 29 inbred strains of mice (2008)

Xing S, Korstanje R, Paigen B
With: Schultz D, Godfrey D

Xing1_Animal

Acclimation and testing periods

Vendor Mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME)

Ages
6, 12, 20 months

Feed

All mice were fed Lab diet® 5K52 (6% fat, PMI Nutritional International, Bentwood, MO) ad libitum

Water

Drinking water was acidified to pH 2.8 to 3.2 to prevent bacterial growth

Housing
Same-sex mice were housed four per pen in duplex polycarbonate cages (31x31x214cm) equipped with pressurized individually ventilated mouse racks (Thoren Caging Systems Inc.) with HEPA filtered supply and exhaust

Bedding

Steam autoclaved white pine shavings

Photoperiod

12 hours (light) on, 12 hours off; or 12 h light : 12 h dark cycle

Relative humidity ~50%

Temperature

21-23 °C

Health status

The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.

Vendor	Mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME)
Ages	6, 12, 20 months
Feed	All mice were fed Lab diet® 5K52 (6% fat, PMI Nutritional International, Bentwood, MO) ad libitum
Water	Drinking water was acidified to pH 2.8 to 3.2 to prevent bacterial growth
Housing	Same-sex mice were housed four per pen in duplex polycarbonate cages (31x31x214cm) equipped with pressurized individually ventilated mouse racks (Thoren Caging Systems Inc.) with HEPA filtered supply and exhaust
Bedding	Steam autoclaved white pine shavings
Photoperiod	12 hours (light) on, 12 hours off; or 12 h light : 12 h dark cycle
Relative humidity	~50%
Temperature	21-23 °C
Health status	The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.