Xenakis2: Effects of inorganic arsenic exposure on body composition and type 2 diabetes indicators in Diversity Outbred (DO) male mice (2022)

Xenakis JG, Douillet C, Bell TA, Hock P, Farrington J, Liu T, Murphy CEY, Saraswatula A, Shaw GD, Nativio G, Shi Q, Venkatratnam A, Zou F, Fry RC, Stýblo M, Pardo-Manuel de Villena F. An interaction of inorganic arsenic exposure with body weight and composition on type 2 diabetes indicators in Diversity Outbred mice. Mamm Genome. 2022 Dec;33(4):575-589. doi: 10.1007/s00335-022-09957-w. Epub 2022 Jul 11.   PubMed 35819478     FullText


         
Xenakis2 downloads
• Download Xenakis2 project data set     animal data, as uploaded
• Download Xenakis2 animal data matrix     with factor-related expansion applied as necessary
• Download Xenakis2 strain means, SD, N, etc.     one row per strain/sex/measure
• Download Xenakis2 supplementary file, Xenakis2.xlsx     Submitted datasheet
Investigators James G Xenakis       University of North Carolina,  Chapel Hill, NC
Rebecca Fry       University of North Carolina,  Chapel Hill, NC
Miroslav Stýblo       University of North Carolina,  Chapel Hill, NC
Fernando Pardo-Manuel de Villena       University of North Carolina,  Chapel Hill, NC
Participants Douillet C, Bell TA, Hock P, Farrington J, Liu T, Murphy CEY, Saraswatula A, Shaw GD, Nativio G, Shi Q, Venkatratnam A, Zou F
ContactFernando Pardo-Manuel de Villena     fernando@med.unc.edu     Lab web site
Funding Provided ByNIH ES029925, ES028721, ES031007, UNC Nutrition Obesity Research Center grant DK056350 (NIDDK), training grant T32ES007126.
Project type Phenotype strain survey data set
MPD identifiersXenakis2     MPD:1125
Data changelog No updates/corrections.       Initial release date: 02/2023.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
The effects of chronic exposure to inorganic arsenic (iAs), a ubiquitous environmental contaminant, and its association with type 2 diabetes were investigated in a cohort of 75 DO mice. Male mice were exposed to iAs in drinking water (100 ppb) for 26 weeks, after which diabetic surrogate risk indicators such as fasting blood glucose and plasma insulin, and blood glucose and plasma insulin 15 minutes after glucose challenge were measured. Additionally, body composition was measured using magnetic resonance imaging and the concentrations of iAs and its methylated metabolites, in particular iAs speciation, were measured in liver and urine.

Experimental groups in this study:
• Inorganic arsenic (iAs)    

Procedures conducted:
• body weight  Baseline, then after iAs exposure for 26 wks
• intake monitoring  Food and water consumption, at baseline then at 1 to 4 wk intervals after iAs exposure
• MRI  Fat and lean tissue mass at baseline, and 12 and 24 wks after iAs exposure
• urinalysis  Urine volume at baseline, and 12 and 24 wks after iAs exposure
• metabolic panel  Blood glucose following 6 h fast, and after intraperitoneal glucose injection; at baseline and after iAs exposure
• hormone quantification  Blood insulin following 6 h fast, and after intraperitoneal glucose injection; at baseline and after iAs exposure
• metabolite quantification  iAs quantification in urine and liver, at 24 and 26 wks after iAs exposure. IAs speciation evaluated specifically monomethyl-arsenic acid (MMA) and dimethyl-arsenic acid (DMA)

Mice: DO population   ♂   age 4-34 wks   1 experimental group