MPD: Data set: Wittenburg3

Wittenburg3: Plasma lipids, cholesterol gallstones, and related gallstone phenotypes in an intercross between DBA/2J and PERA/EiJ (2005)

References (chronological)

1. Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining data from multiple inbred line crosses improves the power and resolution of quantitative trait loci mapping. Genetics. 2005 Mar;169(3):1699-709. Epub 2005 Jan 16. PubMed 15654110 FullText

2. Wittenburg H, Lyons MA, Li R, Kurtz U, Mössner J, Churchill GA, Carey MC, Paigen B. Association of a lithogenic Abcg5/Abcg8 allele on Chromosome 17 (Lith9) with cholesterol gallstone formation in PERA/EiJ mice. Mamm Genome. 2005 Jul;16(7):495-504. PubMed 16151694

3. Wittenburg H, Lyons MA, Li R, Kurtz U, Wang X, Mössner J, Churchill GA, Carey MC, Paigen B. QTL mapping for genetic determinants of lipoprotein cholesterol levels in combined crosses of inbred mouse strains. J Lipid Res. 2006 Aug;47(8):1780-90. Epub 2006 May 9. PubMed 16685081

Wittenburg3 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.

Ontology terms mapped to Wittenburg3 measures:

MA:0000356 gall bladder
MA:0000358 liver
MA:0002501 plasma
MA:0002513 bile
MP:0000180 abnormal circulating cholesterol level
MP:0000181 abnormal circulating LDL cholesterol level
MP:0000184 abnormal circulating HDL cholesterol level
MP:0001259 abnormal body weight
MP:0004773 abnormal bile composition
MP:0004847 abnormal liver weight
MP:0020131 abnormal gallbladder volume
VT:0000180 blood cholesterol amount
VT:0000181 blood LDL cholesterol amount
VT:0000184 blood HDL cholesterol amount
VT:0001259 body mass
VT:0003402 liver mass
VT:0005084 gall bladder morphology trait
VT:0005675 gall bladder size trait

Investigators	H Wittenberg Gary A Churchill The Jackson Laboratory, Bar Harbor, ME
Participants	Lyons MA, Li R, Kurtz U, Mössner J, Carey MC, Paigen B
Contact	Gary A Churchill gary.churchill@jax.org
Affiliated Center	QTL Archive
Acknowledgements	Funding provided by DK51568, DK36588, DK52911, DK34854, DK73687, GM70683, HL55001; and the American Physiological Society, American Liver Foundation, National Health and Medical Research Council of Australia, the Gastroenterological Society of Australia and Deutsche Forschungsgemeinschaft.
Project type	QTL Archive data set
MPD identifiers	Wittenburg3 MPD:182
Data changelog	No updates/corrections. Initial release date: 01/2008.

An intercross between DBA/2J and PERA/EiJ was generated to identify QTLs for cholesterol gallstone susceptibility. At 6-8 wks of age, backcross progeny of both sexes were administered a high-fat (15%) diet for 8 wks and examined for gallstone formation and related phenotypes. Mice were fasted prior to sample collection. For detailed protocols see Wittenburg et al 2005.

QTL data set parameters:

Progenitor strains	DBA/2J PERA/EiJ
Cross direction notation	(PERAxDBA) F1 x (PERAxDBA) F1; (DBAxPERA) F1 x (DBAxPERA) F1
Cross type	F2 intercross
Population size	166 ♀ 158 ♂
Age	14-16wks

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Wittenburg3	body weight	high-fat diet	body weight [g] bw 18201	QTL population	both	14-16wks	N=162

Wittenburg3	organ weights	high-fat diet	liver weight [g] liver_wt 18202	QTL population	both	14-16wks	N=162

Wittenburg3	organ weights	high-fat diet	percentage of body weight that is liver [%] pct_liver_wt 18203	QTL population	both	14-16wks	N=162

Wittenburg3	organ dimensions	high-fat diet	gallbladder volume, log transformed [log(µL)] gallbladder_vol_log 18204	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallstones, presence (score 0=absent, 1=present) [score] gallstone_presence 18205	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	sandy gallstones, presence (score 0=absent, 1=present) [score] gallstone_sandy 18206	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	solid gallstones, presence (score 0=absent, 1=present) [score] gallstone_solid 18207	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	number of gallstones [n] gallstone_count 18208	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallstone weight [mg] gallstone_wt 18209	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallbladder, cholesterol monohydrate crystals (score 0=absent, 1-5=severity) [score] gallstone_crystals 18210	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallbladder, mucin (score 0=absent, 1-5=severity) [score] gallbladder_mucin 18211	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallstone score (score 0=absent, 1=crystals only, 2=sandy or solid gallstones) [score] gallstone_score 18212	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallstone severity index (score 0-3, increasing severity) [score] gallstone_severity 18213	QTL population	both	14-16wks	N=162

Wittenburg3	lipid profile	high-fat diet	total cholesterol (plasma), log transformed [log(mg/dL)] chol_log 18214	QTL population	both	14-16wks	N=162

Wittenburg3	lipid profile	high-fat diet	HDL cholesterol (plasma) [mg/dL] HDL 18215	QTL population	both	14-16wks	N=162

Wittenburg3	lipid profile	high-fat diet	HDL cholesterol (plasma), log transformed [log(mg/dL)] HDL_log 18216	QTL population	both	14-16wks	N=162

Wittenburg3	lipid profile	high-fat diet	nonHDL cholesterol (plasma), log transformed [log(mg/dL)] nonHDL_log 18217	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallstone weight if gallstones present [mg] gallstone_wt_if_present 18218	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	gallstones presence score (0=absent, 1=present) gallstone_present_absent 18219	QTL population	both	14-16wks	N=162

Wittenburg3	histopathology	high-fat diet	Revised subjective summary score (0-3) score_new 18220	QTL population	both	14-16wks	N=162

To see more rows use the paging control at top.

Associated genomic features

MGI-curated QTL / genomic features that have been associated with this data set:
Hdlq10 Hdlq33 Hdlq34 Hdlq35 Hdlq36 Hdlq37 Hdlq38 Hdlq39 Hdlq83 Hdlq84 Lith12 Lith14 Lith15 Lith16 Lith17 Lith26 Lith27 Lith28 Lith29 Lith4 Lith6 Lith9 Nhdlq10 Nhdlq8 Nhdlq9

Project / data set: Wittenburg3 a.k.a. Wittenburg_2005
Excel file: Data_Description_PERAxDBA_Wittenburg2005.xlsx
CSV file: Wittenburg2005_PERAxDBA_B37_Data.csv
Readme / text file: readme_Wittenburg2005.txt