MPD: Data set: Wittenburg2

Wittenburg2: Cholesterol gallstone formation in reciprocal backcrosses between PERA/EiJ and I/LnJ (2003)

References (chronological)

1. Wittenburg H, Lyons MA, Li R, Churchill GA, Carey MC, Paigen B. FXR and ABCG5/ABCG8 as determinants of cholesterol gallstone formation from quantitative trait locus mapping in mice. Gastroenterology. 2003 Sep;125(3):868-81. PubMed 12949731

2. Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining data from multiple inbred line crosses improves the power and resolution of quantitative trait loci mapping. Genetics. 2005 Mar;169(3):1699-709. Epub 2005 Jan 16. PubMed 15654110 FullText

3. Wittenburg H, Lyons MA, Li R, Kurtz U, Wang X, Mössner J, Churchill GA, Carey MC, Paigen B. QTL mapping for genetic determinants of lipoprotein cholesterol levels in combined crosses of inbred mouse strains. J Lipid Res. 2006 Aug;47(8):1780-90. Epub 2006 May 9. PubMed 16685081

Wittenburg2 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.

Ontology terms mapped to Wittenburg2 measures:

MA:0000356 gall bladder
MA:0000358 liver
MA:0002501 plasma
MA:0002513 bile
MP:0000180 abnormal circulating cholesterol level
MP:0000181 abnormal circulating LDL cholesterol level
MP:0000184 abnormal circulating HDL cholesterol level
MP:0001259 abnormal body weight
MP:0004773 abnormal bile composition
MP:0004847 abnormal liver weight
MP:0020131 abnormal gallbladder volume
VT:0000180 blood cholesterol amount
VT:0000181 blood LDL cholesterol amount
VT:0000184 blood HDL cholesterol amount
VT:0001259 body mass
VT:0003402 liver mass
VT:0005084 gall bladder morphology trait
VT:0005675 gall bladder size trait

Investigators	H Wittenberg Gary A Churchill The Jackson Laboratory, Bar Harbor, ME
Participants	Lyons MA, Li R, Carey MC, Paigen B
Contact	Gary A Churchill gary.churchill@jax.org
Affiliated Center	QTL Archive
Acknowledgements	Funding provided by NIH DK51568, DK36588, DK52911, DK34854, CA34196, DK73687, GM070683, HL55001; Deutsche Forschungsgemeinschaft, American Physiological Society, and the American Liver Foundation, the National Health and Medical Research Council of Australia, the Gastroenterological Society of Australia.
Project type	QTL Archive data set
MPD identifiers	Wittenburg2 MPD:138
Data changelog	No updates/corrections. Initial release date: 01/2008.

An intercross of PERA/EiJ and I/LnJ was generated to identify cholesterol gallstone susceptibility loci. F2 progeny of both sexes were administered a high-fat (15%) diet for 8 wks and examined for gallstone formation and related phenotypes. Gallstones were characterized qualitatively and quantitatively, and lipid profiles determined for plasma samples. Mice were fasted prior to sample collection. For detailed protocols see Wittenburg et al 2003.

QTL data set parameters:

Progenitor strains	I/LnJ PERA/EiJ
Cross type	F2 intercross
Population size	164 ♀ 141 ♂

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Wittenburg2	body weight	(none)	body weight, 6-8wks of age [g] bw_start 13801	QTL population	both	6-8wks	N=152

Wittenburg2	body weight	high-fat diet	body weight change (bw end minus bw start), 14-16wks of age [g] bw_change 13802	QTL population	both	14-16wks	N=152

Wittenburg2	organ weights	high-fat diet	liver weight [g] liver_wt 13803	QTL population	both	14-16wks	N=152

Wittenburg2	organ weights	high-fat diet	percentage of total body weight that is liver weight [%] liver_wt_pct 13804	QTL population	both	14-16wks	N=152

Wittenburg2	organ dimensions	high-fat diet	gallbladder, volume [µL] gallbladder_vol 13805	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	gallbladder, mucin (score 0-5) [score] gallbladder_mucin 13806	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	gallstones, presence (score 0=absent, 1=present) [score] gallstone_presence 13807	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	gallstones, screening (score 0=absent, 1=present, 2=solid gallstones) [score] gallstone_screen 13808	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	number of solid gallstones [n] gallstone_count 13809	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	weight of solid gallstones [mg] gallstone_wt 13810	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	weight of solid gallstones, log transformed [log(mg)] gallstone_wt_log 13811	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	weight of solid gallstones ([mg] + 1), log10 transformed [log(mg)] gallstone_wt_log10 13812	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	weight of solid gallstones, square root [sqrt(mg)] gallstone_wt_sqr 13813	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	gallstones, presence of sandy stones (score 0=absent, 1=present) [score] gallstone_sandy 13814	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	gallbladder, cholesterol monohydrate crystals (score 0-5) [score] gallstone_crystals 13815	QTL population	both	14-16wks	N=152

Wittenburg2	histopathology	high-fat diet	gallstone severity (score 0-3) [score] gallstone_score 13816	QTL population	both	14-16wks	N=152

Wittenburg2	lipid profile	high-fat diet	total cholesterol (plasma) [mg/dL] chol 13817	QTL population	both	14-16wks	N=152

Wittenburg2	lipid profile	high-fat diet	HDL (plasma) [mg/dL] HDL 13818	QTL population	both	14-16wks	N=152

Wittenburg2	lipid profile	high-fat diet	HDL (plasma), log transformed [log(mg/dL)] HDL_log 13819	QTL population	both	14-16wks	N=152

Wittenburg2	lipid profile	high-fat diet	nonHDL (plasma) [mg/dL] nonHDL 13820	QTL population	both	14-16wks	N=152

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Associated genomic features

MGI-curated QTL / genomic features that have been associated with this data set:
Hdlq10 Hdlq33 Hdlq34 Hdlq35 Hdlq36 Hdlq37 Hdlq38 Hdlq39 Lith7 Lith8 Lith9 Nhdlq10 Nhdlq8 Nhdlq9

Project / data set: Wittenburg2 a.k.a. Wittenburg_2003
Excel file: Data_Description_PERAxI_Wittenburg2003.xlsx
CSV file: Wittenburg2003_PERAxI_B37_Data.csv
Readme / text file: readme_Wittenburg2003.txt