Sugiyama1 project protocol

Blood pressure survey in males of 25 inbred strains of mice   (2007)

Sugiyama F, Tsukahara C, Paigen B
With: Kunita S, Yagami K




Sugiyama1 Protocol

Project protocol — Contents
 Workflow and sampling
Equipment
Reagents, supplies, and solutions
Procedure: Blood pressure and heart rate measurement using BP-98A
Data
References

Workflow and sampling

 Workflow

Day
Procedure done
Time
Data collected
1-5
 1st to 5th day of training on blood pressure machine
9-12 a.m.
 None
6
 1st day of blood pressure and heart rate measurement
9-12 a.m.
 Blood pressure and heart rate (20 readings)
7
 2nd day of blood pressure and heart rate measurement
9-12 a.m.
 Blood pressure and heart rate (20 readings)
8
 3rd day of blood pressure and heart rate measurement
9-12 a.m.
 Blood pressure and heart rate (20 readings)
9
 4th day of blood pressure and heart rate measurement
9-12 a.m.
 Blood pressure and heart rate (20 readings)
10
 5th day of blood pressure and heart rate measurement
9-12 a.m.
 Blood pressure and heart rate (20 readings)

Equipment and supplies

Blood pressure machine, BP-98A  (Softron, Tokyo, Japan)

Reagents and solutions

Medical tape, paper towels, ethanol (70%)

Acclimation to test conditions

In order for the mice to become accustomed to the testing protocol, they are trained initially on the blood pressure machine for 5 days without data collection.

Procedure: Blood pressure and heart rate measurement using BP-98A

a. The same time of day (9-12 a.m.) is chosen to test the mice in order to avoid normal daily variation in blood pressure.
b. The experiment is run for 10 consecutive days (5 days of training and 5 days of measurements).
c. To optimize cardiovascular circulation, the mice are kept warm at 37°C within a cylindrical heater, with the exception of the tail, and further wrapped in an inner cover of cotton sheet.
d. A programmable sensor attached to a tail (or inflatable balloon) cuff is used to monitor for tail pulse waves and to measure blood pressure and heart rate when the pulse waves become stable and rhythmic.
e. Blood pressure and heart rate measurements are read and recorded via a computer.
f. Following 5 days of training, an average of 100 readings (or 20 readings for each day of the 5 consecutive days of testing) per mouse is used.
g. After testing the mice are gently picked up via their tail and quietly returned into their cages. As much as possible, the whole experience should be as relaxing, and least stressful for the mice.
h. After each mouse is tested, the testing area is cleaned up in preparation for the next mouse to be tested. Urine puddles and fecal boli are discarded from the testing platform using paper towel wet with a disinfectant and the area is further wiped with alcohol, and completely dried.

Investigator's note: Krege et al. (1995) reported that the blood pressure from individual mice by tail-cuff and intra-arterial methods are highly correlated.

Data collected by investigator

Blood pressure and tail pulse (heart rate)



References

    Krege JH, Hodgin JB, Hagaman JR, Smithies O. A noninvasive computerized tail-cuff system for measuring blood pressure in mice. Hypertension. 1995 May;25(5):1111-5. PubMed 7737724