MPD: Data set: Schughart3

Schughart3: Susceptibility to influenza A (H3N2) viral infection: body weight and survival at three doses in the 8 inbred founder strains of the Collaborative Cross (2013)

Leist SR, Pilzner C, van den Brand JM, Dengler L, Geffers R, Kuiken T, Balling R, Kollmus H, Schughart K. Influenza H3N2 infection of the collaborative cross founder strains reveals highly divergent host responses and identifies a unique phenotype in CAST/EiJ mice. BMC Genomics. 2016 Feb 27;17:143. doi: 10.1186/s12864-016-2483-y. PubMed 26921172 FullText

Schughart3 downloads
• Download Schughart3 project data set animal data, as uploaded
• Download Schughart3 animal data matrix with factor-related expansion applied as necessary
• Download Schughart3 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Schughart3 measures:

Investigators	Sarah R Leist Helmholtz Center for Infection Research, Braunschweig GERMANY Heike Kollmus Helmholtz Center for Infection Research, Braunschweig GERMANY Klaus Schughart Helmholtz Center for Infection Research, Braunschweig GERMANY
Contact	Klaus Schughart Klaus.Schughart@helmholtz-hzi.de Lab web site
Part of a series:	• study 1 (this project) Schughart3 • study 2 see Schughart4 • study 3 see Schughart5
Acknowledgements	Funding provided by the Helmholtz-Association (Program Infection and Immunity); FluResearchNet (No. 01KI07137) from the German Ministry of Education and Research: Gesellschaft der Freunde der Tierärztlichen Hochschule Hannover
Project type	Phenotype strain survey data set
MPD identifiers	Schughart3 MPD:571
Data changelog	No updates/corrections. Initial release date: 01/2017.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice infected with influenza A H3N2 virus at three doses were analyzed for subsequent daily weight loss and survival over a period of 14 days. The mouse-adapted virus strain influenza A/HK/01/68 was used. Body weight loss is available for days 1 through 14. Overall survival and mortality rates are also available in this project. Viral loads, lung weights and leukocyte profiles from additional cohorts are available from females in Schughart4 and Schughart5.

Procedures conducted:

• body weight		Influenza A (H3N2) virus (several doses) vs. baseline. Several timepoints.
• health assessment		Survival rate, susceptibility, influenza A (H3N2) virus (several doses).

Mice: inbred w/CC8 8 strains ♀♂ age 7-13wks 3 experimental groups

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Schughart3:1	Schughart3	body weight	(none)	body weight pre-infection [g] bw 57101 10 FFU group 2000 FFU group 200000 FFU group	inbred w/CC8	8	both	7-13wks	N=11

Schughart3:11	Schughart3	body weight	influenza A (H3N2) virus	body weight as percent of baseline, 10 FFU group [%] pctbwA 57111 days post-infection: 1 2 3 4 5 6 7 8 9 10 11 12 13 14	inbred w/CC8	8	both	7-13wks	N=9

Schughart3:31	Schughart3	body weight	influenza A (H3N2) virus	body weight as percent of baseline, 2000 FFU group [%] pctbwB 57131 days post-infection: 1 2 3 4 5 6 7 8 9 10 11 12 13 14	inbred w/CC8	8	both	7-13wks	N=9

Schughart3:51	Schughart3	body weight	influenza A (H3N2) virus	body weight as percent of baseline, 200,000 FFU group [%] pctbwC 57151 days post-infection: 1 2 3 4 5 6 7 8 9 10 11 12 13 14	inbred	2	both	7-13wks	N=12

Schughart3:81	Schughart3	health assessment	influenza A (H3N2) virus	survival, post-infection [d] day_survival 57181 10 FFU group 2000 FFU group 200000 FFU group	inbred w/CC8	8	both	7-13wks	N=11

Schughart3:91	Schughart3	health assessment	influenza A (H3N2) virus	susceptibility to viral infection (0=resistant, 1=susceptible), mortality rate [score] status_day_survival 57191 10 FFU group 2000 FFU group 200000 FFU group	inbred w/CC8	8	both	7-13wks	N=11

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