MPD: Data set: Rusyn5

Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Rusyn I. Interstrain differences in liver injury and one-carbon metabolism in alcohol-fed mice. Hepatology. 2012 Jul;56(1):130-9. doi: 10.1002/hep.2564Epub 2012 Jun 6. PubMed 22307928 FullText

Rusyn5 downloads
• Download Rusyn5 project data set animal data, as uploaded
• Download Rusyn5 animal data matrix with factor-related expansion applied as necessary
• Download Rusyn5 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Rusyn5 measures:

MA:0000059 blood
MA:0000358 liver
MA:0002501 plasma
MA:0002502 serum
MA:0002545 urine
MP:0000598 abnormal liver morphology
MP:0000609 abnormal liver physiology
MP:0001573 abnormal circulating alanine transaminase level
MP:0001654 hepatic necrosis
MP:0004847 abnormal liver weight
MP:0005332 abnormal amino acid level
MP:0005443 abnormal ethanol metabolism
MP:0006076 abnormal circulating homocysteine level
MP:0008469 abnormal protein level
MP:0011601 abnormal glutathione peroxidase activity
MP:0012778 abnormal liver triglyceride level
VT:0000187 triglyceride amount
VT:0000598 liver morphology trait
VT:0000609 liver physiology trait
VT:0001573 blood alanine transaminase amount
VT:0002136 kidney physiology trait
VT:0003402 liver mass
VT:0005443 ethanol metabolism trait
VT:0006076 blood homocysteine amount
VT:0010120 protein amount
VT:0010489 response to alcohol trait
VT:0010516 blood glutathione amount
VT:0015078 blood homeostasis trait

Investigators	Ivan Rusyn Texas A&M University, College Station, TX
Participants	Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Gatti DM, Kawaguchi A, Lam TC, Uehara T, Wright FA
Contact	Ivan Rusyn IRusyn@cvm.tamu.edu Lab web site
Acknowledgements	Funding provided by NIH AA016258, AA014428, AA018846, ES010126, ES015241
Project type	Phenotype strain survey data set
MPD identifiers	Rusyn5 MPD:430
Data changelog	2 updates/corrections. Initial release date: 07/2012.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

The effects of high-fat diet on alcohol-induced liver injury were assessed using an intragastric intubation model. Urine alcohol content was monitored daily. Mice were evaluated for endoplasmic reticulum stress response as well as lipid and one-carbon metabolism. There were 2 cohorts: (1) mice fed high-fat diet only; (2) mice fed high-fat diet plus alcohol.

Procedures conducted:

• metabolic panel		Alanine transaminase (serum) after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• alcohol quantification		Alcohol concentration in blood and urine after 4 wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• organ weights		Liver weight after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• histopathology		Liver histopathology, injury after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• metabolite quantification		Homocysteine concentration after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• lipid profile		Hepatic triglycerides after 4wk regimen of ethanol and high-fat diet. 24h fast.
• RNA expression profiling		Liver gene expression after 4wk regimen of ethanol and high-fat diet. 24h fast. mRNA abundance relative to the control (high-fat only) group.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Rusyn5:1	Rusyn5	metabolic panel	high-fat diet and ethanol	serum alanine transaminase (ALT) [IU/L] ALT 43001 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:3	Rusyn5	metabolite quantification	high-fat diet and ethanol	homocysteine concentration (plasma) [nmol/mL] plasma_homocysteine 43003 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:5	Rusyn5	alcohol quantification	high-fat diet and ethanol	blood alcohol concentration (BAC) [mg/dL] BAC 43005 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:7	Rusyn5	alcohol quantification	high-fat diet and ethanol	urine alcohol concentration (UAC), daily average [mg/dL] avg_UAC 43007 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:9	Rusyn5	alcohol quantification	high-fat diet and ethanol	urine alcohol concentration (UAC), daily peak [mg/dL] peak_UAC 43009 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:11	Rusyn5	organ weights	high-fat diet and ethanol	liver weight as a percent of body weight [%] pctLW_BW 43011 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:13	Rusyn5	histopathology	high-fat diet and ethanol	liver steatosis (0-4) [score] steatosis 43013 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:15	Rusyn5	histopathology	high-fat diet and ethanol	liver inflammation (0-2) [score] inflammation 43015 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:17	Rusyn5	histopathology	high-fat diet and ethanol	liver necrosis (0-2) [score] necrosis 43017 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:19	Rusyn5	histopathology	high-fat diet and ethanol	total liver histopathology (0-8) [score] total_pathology 43019 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:21	Rusyn5	lipid profile	high-fat diet and ethanol	hepatic triglycerides (TG) [mg/g] liver_TG 43021 high-fat only high-fat and ethanol	inbred	6	m	8-14wks	N=3

Rusyn5:23	Rusyn5	metabolite quantification	high-fat diet and ethanol	reduced liver glutathione (GSH) [nmol/mg] liver_GSH 43023 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:25	Rusyn5	metabolite quantification	high-fat diet and ethanol	oxidized liver glutathione (GSSG) [nmol/mg] liver_GSSG 43025 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:29	Rusyn5	metabolite quantification	high-fat diet and ethanol	ratio of reduced to oxidized liver glutathione [ratio] liver_GSHratio 43029 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:31	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver homocysteine [nmol/mg] liver_homocysteine 43031 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:33	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver S-adenosylhomocysteine (SAH) [nmol/mg] liver_SAH 43033 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:35	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver methionine [nmol/mg] liver_methionine 43035 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:37	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver S-adenosylmethionine (SAM) [nmol/mg] liver_SAM 43037 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:39	Rusyn5	metabolite quantification	high-fat diet and ethanol	ratio of liver SAM to SAH (SAM/SAH) [ratio] liver_SAM_SAH 43039 high-fat only high-fat and ethanol	inbred	14	m	8-14wks	N=3

Rusyn5:42	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver BHMT, protein expression relative to high-fat-only group [%] liver_BHMT_hfe 43042	inbred	14	m	8-14wks	N=3

Rusyn5:44	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver DDIT3 (formerly CHOP), protein expression relative to high-fat-only group [%] liver_DDIT3_hfe 43044	inbred	14	m	8-14wks	N=3

Rusyn5:46	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver HSPA5 (formerly GRP78), protein expression relative to high-fat-only group [%] liver_HSPA5_hfe 43046	inbred	14	m	8-14wks	N=3

Rusyn5:48	Rusyn5	metabolite quantification	high-fat diet and ethanol	liver SREBF1 (formerly NSREBP1), protein expression relative to high-fat-only group [%] liver_SREBF1_hfe 43048	inbred	14	m	8-14wks	N=3

Rusyn5:50	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Acox1, mRNA abundance relative to high-fat-only group [%] liver_Acox1_hfe 43050	inbred	6	m	8-14wks	N=3

Rusyn5:52	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Adipor2, mRNA abundance relative to high-fat-only group [%] liver_Adipor2_hfe 43052	inbred	6	m	8-14wks	N=3

Rusyn5:54	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Ahcy, mRNA abundance relative to high-fat-only group [%] liver_Ahcy_hfe 43054	inbred	14	m	8-14wks	N=3

Rusyn5:56	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Cbs, mRNA abundance relative to high-fat-only group [%] liver_Cbs_hfe 43056	inbred	14	m	8-14wks	N=3

Rusyn5:58	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Cebpa, mRNA abundance relative to high-fat-only group [%] liver_Cebpa_hfe 43058	inbred	14	m	8-14wks	N=3

Rusyn5:60	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Cth, mRNA abundance relative to high-fat-only group [%] liver_Cth_hfe 43060	inbred	14	m	8-14wks	N=3

Rusyn5:62	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Ddit3 (formerly Chop), mRNA abundance relative to high-fat-only group [%] liver_Ddit3_hfe 43062	inbred	14	m	8-14wks	N=3

Rusyn5:64	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Gnmt, mRNA abundance relative to high-fat-only group [%] liver_Gnmt_hfe 43064	inbred	14	m	8-14wks	N=3

Rusyn5:66	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Mat1a, mRNA abundance relative to high-fat-only group [%] liver_Mat1a_hfe 43066	inbred	14	m	8-14wks	N=3

Rusyn5:68	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Mthfr, mRNA abundance relative to high-fat-only group [%] liver_Mthfr_hfe 43068	inbred	14	m	8-14wks	N=3

Rusyn5:70	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Mtr, mRNA abundance relative to high-fat-only group [%] liver_Mtr_hfe 43070	inbred	14	m	8-14wks	N=3

Rusyn5:72	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Mttp, mRNA abundance relative to high-fat-only group [%] liver_Mttp_hfe 43072	inbred	6	m	8-14wks	N=3

Rusyn5:74	Rusyn5	RNA expression profiling	high-fat diet and ethanol	liver Srebf1, mRNA abundance relative to high-fat-only group [%] liver_Srebf1_hfe 43074	inbred	14	m	8-14wks	N=3

Rusyn5: Drug study: High-fat diet plus subchronic alcohol-induced effects in males of 14 inbred strains of mice (2011)

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