Project protocol - Contents

• Workflow and sampling
• Equipment and supplies
• Procedure: Body weight and length, organ weights
• Procedure: Body composition and bone mineral content and density
• Data

Workflow and sampling

Step	Procedure	Equipment	Data collected
1	Mice euthanized, weighed and measured	Scales, ruler, and electronic calipers	Body weight, length
2	Organs and fat pads harvested and weighed	Scales	Weights of selected organs and fat pads
3	Dual-energy X-ray absorptiometry (DXA)	Densiotometer (Lunar PIXImus II)	Body composition, bone area, bone mineral content and density

Equipment and supplies

• Scales
• Electronic calipers (Fowler ProMax, Kelley and Kelley Industrial Supply, Syracuse NY)
• Lunar PIXImus II densiotometer (GE, software v 2.0., Lunar Corp, Madison WI)

Procedure: Body weight and length, organ weights

1. Mice are euthanized and weighed using scales.
2. Mice are measured for length using either a ruler or electronic calipers (bottom of incisors to anus).
3. Organs are harvested and weighed (brain, heart, kidneys, liver, pancreas, spleen).

Procedure: Body composition and bone mineral content and density

1. Mice are subjected to DXA (head excluded).
2. Bone area, fat and lean weights, bone minerals, and bone mineral density are measured.

Data collected by investigator

• Body weight and length
• Organ weights
• Fat pad weights
• Body composition
• Bone area
• Bone mineral content and density