Procedures

Procedure 1: Diet administration

Reagents and solutions

• Standard chow diet (Purina LabDiet, 5010, St. Louis, MO, USA)
• High-fat High-sucrose (HFHS) diet (Research Diets, D12331, New Brunswick, NJ, USA)

Steps

1. All mice are fed the chow diet until 4-5 weeks of age.
2. At 4-5 wks of age, all mice are fed the HFHS diet until the end of the study at 18-19 weeks of age.

Procedure 2: Body weight and size determination

Equipment, software, and supplies

• Balance scale
• Measuring ruler

Reagents and solutions

• Avertin, 0.8 mg/g of 2,2,2,-tribromoethanol in tertiary-amyl alcohol (Fisher Scientific, Pittsburgh, PA, USA)

Steps

1. Mice are weighed on a weekly basis 4-5 wks of age up to 18-19 wks of age.
2. Mice at 18-19 wks of age are moved to clean cages, fasted overnight, weighed, and then anesthetized via intraperitoneal injection with Avertin. Nose to anus length is measured to calculate BMI.