MPD: Data set: Mrug1

Mrug1: Identification of polycystic kidney disease modifier candidate in cpk mutant mice (2005)

Mrug M, Li R, Cui X, Schoeb TR, Churchill GA, Guay-Woodford LM. Kinesin family member 12 is a candidate polycystic kidney disease modifier in the cpk mouse. J Am Soc Nephrol. 2005 Apr;16(4):905-16. Epub 2005 Feb 23. PubMed 15728779

The cpk mutant mouse is the most characterized model of autosomal recessive polycystic kidney disease. To identify the modifier that modulates disease severity, an intercross between B6-+/cpk (heterozygous for the Cys1^cpk mutation) and CAST was generated and 461 F2 cpk homozygous mice were examined for renal phenotypes. A major contributed QTL is mapped on chromosome 4 and gene Kif12 is found in this region. This gene is proposed as a cpk modifier gene of renal disease. For detailed protocols see Mrug et al 2005.

Note on studied population: cpk mutant mice

Procedures conducted:

• study archive

Kidney morphology.

Mrug1 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.

Ontology terms mapped to Mrug1 measures:

MA:0000358 liver
MA:0000368 kidney
MP:0001259 abnormal body weight
MP:0001845 abnormal inflammatory response
MP:0002135 abnormal kidney morphology
MP:0002706 abnormal kidney size
MP:0002707 abnormal kidney weight
MP:0009494 abnormal biliary ductule morphology
VT:0001259 body mass
VT:0002706 kidney size trait
VT:0002707 kidney mass
VT:0005083 biliary tract morphology trait
VT:0010139 immune response trait

Investigators	M Mrug Gary A Churchill The Jackson Laboratory, Bar Harbor, ME
Participants	Li R, Cui X, Schoeb TR, Guay-Woodford LM
Contact	Gary A Churchill gary.churchill@jax.org
Affiliated Center	QTL Archive
Acknowledgements	This work was supported by a Polycystic Kidney Disease Foundation Fellowship Award (M.M.), a Clinical Scientist Award in Translational Research from the Burroughs-Wellcome Foundation (L.M.G.-W.), and the National Institutes of Health (G.A.C.).
Project type	QTL Archive data set
MPD identifiers	Mrug1 MPD:175
Data changelog	No updates/corrections. Initial release date: 08/2011.

QTL data set parameters:

Progenitor strains	C57BL/6J-+/cpk CAST/EiJ
Cross type	F2 intercross
Population size	209 ♀ 251 ♂
Notes	the sex information of 1 mouse is missing

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Mrug1	histopathology	(none)	mean of biliary duct number [n] mean_BDN 17501	QTL population	both	10d	N=230

Mrug1	histopathology	(none)	mean of log(biliary duct number) [log(n)] mean_logBDN 17502	QTL population	both	1wk	N=230

Mrug1	histopathology	(none)	cholangitis severity (score 0-4, increasing severity) 0 = no/minimal inflammation, 4=severe and extensive cholangiohepatitis [score] Cholangitis 17503	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	kidney length [mm] Klength 17504	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	kidney length, square root [sqrt(mm)] sqrtKL 17505	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	crown to rump length of kidney [mm] CRLength 17506	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	kidney weight/crown to rump length KL-CRL-Ratio 17507	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	kidney weight/crown to rump length, log transformed logKL-CRL-Ratio 17508	QTL population	both	10d	N=230

Mrug1	organ weights	(none)	kidney weight [g] KW 17509	QTL population	both	10d	N=230

Mrug1	organ weights	(none)	kidney weight, square root [sqrt(g)] sqrtKW 17510	QTL population	both	10d	N=230

Mrug1	body weight	(none)	mouse body weight [g] BodyWeight 17511	QTL population	both	10d	N=230

Mrug1	organ weights	(none)	kidney weight/body weight KW-BW-Ratio 17512	QTL population	both	10d	N=230

Mrug1	organ weights	(none)	kidney weight/body weight, log transformed logKW-BW-ratio 17513	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	kidney volume [ml] KVolume 17514	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	kidney volume, square root [sqrt(ml)] sqrtKV 17515	QTL population	both	10d	N=230

Mrug1	organ dimensions	(none)	renal principal component of kidney length, weight and volume Prin1 17516	QTL population	both	10d	N=230

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Associated genomic features

MGI-curated QTL / genomic features that have been associated with this data set:
Mpdk1 Mpdk2 Mpdk3 Mpdk4 Mpdk5 Mpdk6 Mpdk7 Mpdk8

Project / data set: Mrug1 a.k.a. Mrug_2005
Excel file: Data_Description_B6xCAST_Mrug2005.xlsx
CSV file: Mrug2005_B6xCAST_B37_Data.csv
Readme / text file: readme_Mrug2005.txt
QC plots, figures, etc.: QCReport_Mrug2005.pdf