Project protocol - Contents

• Workflow and sampling
• Equipment and supplies
• Reagents and solutions
• Procedure: Olanzapine preparation and administration
• Procedure: Body weights
• Data

Workflow and sampling

Step	Procedure	Equipment	Data collected *
1	Body weight measured at 7 wks of age (pre-treatment)	Scales	Body weight
2	Mice placed on high-fat diet with or without olanzapine at 8 wks of age	-	-
3	Mice weighed weekly from 8-18 wks of age	Scales	Body weight

*Supplementary data are available for this project. See Morgan1

Equipment and supplies

• Scales

Reagents and solutions

• Olanzapine

Procedure: Olanzapine preparation and administration

1. Olanzapine is compounded into high-fat food at a concentration of 50 mg/kg of diet by Research Diets, Inc. (D0909293; 45 kcal% fat).
2. Control animals receive the same high-fat diet, minus olanzapine, prepared in parallel to control for batch effects.

Procedure: Body weights

1. Mice are weighed weekly, starting at 7 wks of age (pre-treatment). Weight at 8 wks of age is considered baseline for percent change in body weight.

Data collected by investigator

• body weight (control and treated mice)

Percent change in body weight per animal computed by MPD

*Supplemental data (body weights at 7-18 wks) are available for this project. See Morgan1