Acclimation & testing periods
VendorMice were purchased in two to six lots per strain, either from Charles River, France (strains Balb/cByJ, C3H/HeOuJ, CBA/J, and DBA/2J) or from The Jackson Laboratory JAX® (Bar Harbor, ME). AgesMales 7-13 wks of age on the day of sacrifice FeedCommercial chow diet, ad libitum (Provimi Kliba AG, Switzerland) WaterTap water, ad libitum Housing4-5 mice/cage Relative humidity30-60% Room temperature25°C BeddingWood chips, saw dust Photoperiod12h/12h light/dark cycle (6:00 A.M. to 6:00 P.M.) Health statusAll animal procedures have been approved by the "Service vétérinaire cantonal vaudois" (authorization nu 1649) and were performed in accordance with the National Institutes of Health (NIH) guidelines for the care and use of laboratory animals. Sentinel mice were periodically examined throughout the study and were free of microbiological infections.