Welcome! Our website uses cookies exclusively for usage analytics and reporting metrics to our stakeholders
and funding agencies about site usage. These cookies also help us understand how visitors interact with our
site, enabling us to improve your browsing experience and the content we offer. We respect your privacy and
ensure that all data collected is anonymized and used solely to guide our enhancements. Additionally, we do
not use this data for marketing purposes nor do we share any user information with third parties.
By clicking "Accept," you consent to our use of cookies for these purposes. For more detailed information on
the cookies we use and how we handle your data, please review our Privacy Policy.
Jaxpheno20:
Multi-system assessment of NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (2018)
Jackson Laboratory
Andrew J Schile
Jaxpheno20 downloads
Click on "Download" link or button below, to see the available downloads for this project.
Institutional authorship
Jackson Laboratory
Investigators
Andrew J Schile The Jackson Laboratory -- West, Sacramento, CA
No updates/corrections.
Initial release date: 12/2018.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
Purpose:
To
characterize NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ male and female mice fed a 6% fat diet (LabDiet® 5K52) between the ages of 3 and 15 weeks (males) or 24 weeks (females).Per-animal data are available in the download file.
Methods
Two hundred eighty male and two hundred eighty female mice were obtained at weaning (birthdate +/- 3 days) from seven rooms from the Bar Harbor and Sacramento facilities of The Jackson Laboratory, and weighed weekly until the oldest age held in inventory. The mice were maintained on a diet containing 6% fat (LabDiet® 5K52 formulation).
At 8, 16 and 24 weeks cohorts of 18-20 mice were weighed and body composition was analyzed using a Lunar PIXImus DEXA scanner. Calculations of body composition excluded the head. Cardiac blood was collected from at least 19 mice per age and sex under isoflurane anesthesia and divided for hematology (using a Siemens Advia 2120 or Drew Scientific Hemavet 950 FS analyzer) or serum biochemistry (Beckman AU680 analyzer). Â Single-cell suspensions were also analyzed by flow cytometry following a protocol found here.
Available
download (per animal data):
Body weights, DXA body composition, spleen flow cytometry, serum clinical chemistry, and hematology.