See JAX Pipeline on the IMPC website for order of tasks for all mice tested

Project protocol: Urinalysis

• Introduction
• Study design
• Workflow
• Equipment, software and supplies
• Reagents and solutions
• Procedure
• Measurements
• Experimental and technical parameters

Introduction

Urinalysis is performed to assess renal function.

Study design

Paradigm: Baseline study

Panel: Collaborative Cross

Number of strains: 14

Sex: Both

Age: 12-13 wks

Sample size: Five mice per sex from at least two litters

Number of cohorts: 1

Workflow

Step	Description	Equipment	Data collected
1	Urine collection (non-fasting), always in the morning	-	-
2	Urinalysis	Clinical chemistry analyzer	Urine glucose, magnesium level, creatinine, microalbumin, albumin-creatinine ratio

Equipment, software and supplies

• Clinical chemistry analyzer: Beckman Coulter AU 680 [See Experimental and technical parameters]
• 1.5 mL Eppendorf tubes
• Pipettes

Reagents and solutions

Test	Au#	Test Kit #	Calibrator Kit #	QC Kit #	Frequency of Assay
Creatinine	12.CRE	Beckman OSR6178	Beckman DR0091	BioRad 395X (2 levels)	Calibrator and Controls assayed for every day of a test run.
Microalbumin	27.MALB	Beckman OSR6167	Beckman DR3024	BioRad 395X (2 levels)	Calibrator and Controls assayed for every day of a test run.
Magnesium	20.MG	Beckman OSR6789	Beckman DR0090	BioRad 395X (2 levels)	Calibrator and Controls assayed for every day of a test run.
Glucose	92.GLUX	Beckman OSR6121	Beckman DR0090	BioRad 395X (2 levels)	Calibrator and Controls assayed for every day of a test run.

 

Procedure

1. Set up the clinical chemistry analyzer and perform QC analysis of the control reagents in accordance with equipment guidelines.
2. At least 70 µL urine is collected into a microcentrifuge tube (collection is in the morning).
3. Samples are kept on ice until analysis.
4. Samples are analyzed on day of collection, undiluted.

Measurements

LIMS field name	MPD variable name	Description	Units	Series	Primary measurement	Series order	MPD computed	Calculation	Method	Data type
microAlbumin (calculated)	MA	microalbumin (urine MA)	mg/dL						urinalysis	Float
Creatinine	CREA	creatinine (urine CREA)	mg/dL						urinalysis	Float
Albumin to Creatinine ratio	ACR	albumin-creatinine ratio (urine ACR)	mg/dL						urinalysis	Float
Magnesium	urine_Mg	magnesium level (urine Mg)	mg/dL						urinalysis	Float
Glucose	urine_GLU	glucose (urine GLU)	mg/dL						urinalysis	Float

Experimental and technical parameters

Equipment name	Clinical chemistry analyzer
Equipment manufacturer	Beckman Coulter
Equipment model	AU 680
Fasting	None
Anesthesia	None
Method of urine collection	Gentle restraint
Sample status	Fresh (on ice)
Urine dilution	No