GMC19 project protocol

Lung function of females of 8 inbred founder strains of the Collaborative Cross   (2020)

German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Stöger T, Yildirim AÖ, Eickelberg O, Lengger C, Kollmus H, Schughart K




German Mouse Clinic: Phenotyping Pipeline

Lung function: 21-22 wks of age

 

  • Project protocol - Contents

    Workflow and sampling

    Step
    Procedure
    		 Equipment
    Data collected
    1 Body weight Balance Body weight
    2 Mice anesthetized - -
    3 Lung function Pulmonary function device Lung volume, mechanics, compliance, airway resistance

    Equipment and supplies

    • Balance: Ohaus (AV213C)
    • 18-gauge cannula
    • Pulmonary function device: Buxco Research Systems (Fine-Pointe RC System), Wilmington NC
    • Pulmonary function device: Buxco Research Systems (Forced Maneuver System), Wilmington NC

    Reagents and solutions

    • Ketamine
    • Xylazine
    • Cleaning solution

    Procedure: Lung function

      1. Mice are weighed and anesthetized by i.p. injection of ketamine (137 mg/kg BW) and xylazine (6.6 mg/kg BW) (performed in the morning).
      2. The trachea is opened by a small incision and a cannula is inserted and fixated by ligation.
      3. Each mouse is placed in the Fine-Pointe System (heated plethysmograph chamber).
      4. Mice are ventilated at an average rate of 130 breaths per min, and flow and mouth pressure and heart rate are monitored.
      5. After an initial acclimation period of three minutes, a two-minute measurement is performed for resistance and compliance.
      6. Mice are then transferred to the Forced Maneuver System where the rest of the pulmonary measurements are taken.

    Data collected by investigator

    • Body weight
    • Chord compliance
    • Dynamic lung compliance
    • Expiratory reverse volume
    • Forced expiratory volume
    • Functional residual capacity
    • Forced vital capacity
    • Inspiratory capacity
    • Peak expiratory flow
    • Lung resistance
    • Residual volume
    • Total lung capacity
    • Tidal volume
    • Vital capacity
    		•