GMC13 project protocol

Heart function assessed by electrocardiography in 6 inbred founder strains of the Collaborative Cross   (2020)

German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Moreth K, Spielmann N, Bekeredjian R, Kattus H, Lengger C, Kollmus H, Schughart K




German Mouse Clinic: Phenotyping Pipeline

Electrocardiogram (ECG): 14-15 wks of age

 

  • Project protocol - Contents

    Workflow and sampling

    Step
    Procedure
    		 Equipment
    Data collected
    1 Electrocardiogram Electrocardiogram (ECG) Heart rate parameters, duration of intervals, amplitude of signals

    Equipment and supplies

    • Electrocardiogram: Mouse Specifics, Inc. (ECGenie), Framingham MA
    • Software: LabChart ECGenie/eMouse

    Reagents and solutions

    • Cleaning solution

    Procedure: Electrocardiogram (ECG)

      1. Lead plates are snapped into place onto the top of the pre-amplifier tower; the covering is removed to reveal three gel coated pads surrounded by a sticking plate; the plate is covered with the extra cover in the package.
      2. The combined amplifier and the pre-amplifier tower are turned on.
      3. The icon ECG acquisition on the acquisition computer is doubled clicked.
      4. The ECG set up file is opened.
      5. A mouse is placed on the pad, lowering the Red Acrylic Cubby to surround the mouse on 3 sides, discouraging escape.
      6. Acquisition begins by pressing 'Start'.
      7. The reading is stopped after 5-10 min of acquisition; there will be one long reading.
      8. Save the data.
      9. Follow manufacturer's instructions for using the software for data analysis.

    Data collected by investigator

    • Heart rate
    • Heart rate variability
    • Heart rate coefficient of variance
    • Duration of RR interval
    • Duration of PQ interval
    • Duration of PR interval
    • Duration of QRS interval
    • Duration of QT interval
    • Duration of ST interval
    • Duration of QTc interval
    • QT dispersion
    • QT rate-corrected dispersion
    • SR amplitude
    • R amplitude
    • Root mean square of successive differences of neighboring RR intervals (rMSSD)
    • Proportion of number of pairs of successive beat-to-beat intervals that exceed 50 ms (pNN50)
    		•