GMC07 project protocol

Thermal nociception assessed by the hot plate test in 8 inbred founder strains of the Collaborative Cross   (2020)

German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Racz I, Becker L, Zimmer A, Lengger C, Kollmus H, Schughart K




German Mouse Clinic: Phenotyping Pipeline

Hot plate test (nociceptive pain): 11 - 12 wks of age

 

  • Project protocol - Contents

    Workflow and sampling

    Step
    Procedure
    		 Equipment
    Data collected
    1
    		 Mice weighed Balance
    Body weight
    2 Thermal nociception test Hot plate Latency to respond, type of response

    Equipment and supplies

    • Hot plate: TSE GmbH (Hot Plate 602001)
    • Plexiglas wall: Circular, 28-cm diameter, 20-cm high
    • Stopwatch

    Reagents and solutions

    • Cleaning solution

    Procedure: Hot plate test

      1. Mice are individually placed within the Plexiglas walls which rests upright on the hot plate (metal surface maintained at 52 +/- 0.2 °C); all four paws are in contact with the metal surface.
      2. Mice remain on the surface until they show first and second reactions to the hotplate regarded indicative of nociception pathway function: hindpaw lick, hindpaw shake/flutter, jumping, or none.
      3. Cutoff time is 30 s.
      4. After the first two reactions or latest cutoff time, mice are removed promptly and placed in their homecage.
      5. Latency is recorded to the nearest 0.1 s.

    Data collected by investigator

    • Body weight
    • Latency to respond to hotplate: first two reactions (hindpaw lick, hindpaw shake/flutter, jumping, or none)
    • Type of first and second reaction