MPD: Data set: French1

French JE, Gatti DM, Morgan DL, Kissling GE, Shockley KR, Knudsen GA, Shepard KG, Price HC, King D, Witt KL, Pedersen LC, Munger SC, Svenson KL, Churchill GA. Diversity Outbred Mice Identify Population-Based Exposure Thresholds and Genetic Factors that Influence Benzene-Induced Genotoxicity. Environ Health Perspect. 2015 Mar;123(3):237-45. doi: 10.1289/ehp.1408202. Epub 2014 Nov 6. PubMed 25376053 FullText

French1 downloads
• Download French1 project data set animal data, as uploaded
• Download French1 project data set animal data, as uploaded
• Download French1 animal data matrix with factor-related expansion applied as necessary
• Download French1 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to French1 measures:

MA:0000059 blood
MA:0000134 bone marrow
MP:0002424 abnormal reticulocyte morphology
MP:0002447 abnormal erythrocyte morphology
MP:0008872 abnormal physiological response to xenobiotic
VT:0002424 reticulocyte morphology trait
VT:0002447 erythrocyte morphology trait
VT:0010487 response to xenobiotic stimulus trait

Investigators	John E French University of North Carolina, Chapel Hill, NC Daniel M Gatti The Jackson Laboratory, Bar Harbor, ME Karen L Svenson The Jackson Laboratory, Bar Harbor, ME Gary A Churchill The Jackson Laboratory, Bar Harbor, ME
Participants	Morgan DL, Kissling GE, Shockley KR, Knudsen GA, Shepard KG, Price HC, King D, Witt KL, Pedersen LC, Munger SC
Contact	John E French jfren43@email.unc.edu
Funding Provided By	NIH NIEHS/NTP, NIEHS, NCI Intramural Research Programs; NIH GM076468, GM070683, DA021336, DA024845, MH079103; The Jackson Laboratory
Project type	Phenotype strain survey data set
MPD identifiers	French1 MPD:1042
Data changelog	This project has not yet been released.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Diversity Outbred mice were exposed to benzene via inhalation in four dosage groups (0, 1, 10, 100 ppm) for 28 days. The presence of micronuclei in erythrocytes and reticulocytes was determined as a measure of chromosomal damage. Data were collected for peripheral blood and bone marrow. Approximately 20,000 reticulocytes and 10⁶ mature erythrocytes were examined. In addition, the percentage of reticulocytes was determined in approximately 10⁶ mature erythrocytes.

Genotypes are available at DODB (search for French).

Note on studied population: 7th and 8th generations

Experimental groups in this study:
• Benzene 0 ppm • Benzene 1 ppm • Benzene 10 ppm • Benzene 100 ppm

Procedures conducted:

• chromosome instability assessment

Percentages of erythrocytes and reticulocytes that are micronucleated before and after exposure.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
French1:13	French1	chromosome instability assessment	(none)	number of non-micronucleated normochromatic erythrocytes, peripheral blood, pre-exposure (x 10³) [n] pb_NCE_pre 112333 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=150

French1:17	French1	chromosome instability assessment	(none)	number of micronucleated normochromatic erythrocytes, peripheral blood, pre-exposure [n] pb_NCE_MN_pre 112337 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=150

French1:25	French1	chromosome instability assessment	(none)	total number of normochromatic erythrocytes, peripheral blood, pre-exposure (x 10³) [n] pb_NCE_total_pre 112345 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=150

French1:29	French1	chromosome instability assessment	(none)	percentage of red blood cells that are reticulocytes, peripheral blood, pre-exposure [%] pb_RET_pct_pre 112349 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=150

French1:37	French1	chromosome instability assessment	(none)	number of non-micronucleated reticulocytes, peripheral blood, pre-exposure (x 10³) [n] pb_RET_pre 112357 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=150

French1:41	French1	chromosome instability assessment	(none)	number of micronucleated reticulocytes, peripheral blood, pre-exposure [n] pb_RET_MN_pre 112361 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=150

French1:45	French1	chromosome instability assessment	(none)	number of micronucleated reticulocytes, peripheral blood per 1000 reticulocytes, pre-exposure [n] pb_RET_MN_pre_adj 112365 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=150

French1:61	French1	chromosome instability assessment	benzene	number of non-micronucleated normochromatic erythrocytes, peripheral blood, post-exposure (x 10³) [n] pb_NCE_post 112381 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=148

French1:65	French1	chromosome instability assessment	benzene	number of micronucleated normochromatic erythrocytes, peripheral blood, post-exposure [n] pb_NCE_MN_post 112385 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=148

French1:73	French1	chromosome instability assessment	benzene	total number of normochromatic erythrocytes, peripheral blood, post-exposure (x 10³) [n] pb_NCE_total_post 112393 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=148

French1:77	French1	chromosome instability assessment	benzene	percentage of red blood cells that are reticulocytes, peripheral blood, post-exposure [%] pb_RET_pct_post 112397 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=148

French1:85	French1	chromosome instability assessment	benzene	number of non-micronucleated reticulocytes, peripheral blood, post-exposure (x 10³) [n] pb_RET_post 112405 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=148

French1:89	French1	chromosome instability assessment	benzene	number of micronucleated reticulocytes, peripheral blood, post-exposure [n] pb_RET_MN_post 112409 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=148

French1:93	French1	chromosome instability assessment	benzene	number of micronucleated reticulocytes, peripheral blood per 1000 reticulocytes, post-exposure [n] pb_RET_MN_post_adj 112413 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=148

French1:105	French1	chromosome instability assessment	benzene	number of non-micronucleated normochromatic erythrocytes, bone marrow, post-exposure (x 10³) [n] bm_NCE_post 112425 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=142

French1:109	French1	chromosome instability assessment	benzene	number of micronucleated normochromatic erythrocytes, bone marrow, post-exposure [n] bm_NCE_MN_post 112429 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=142

French1:117	French1	chromosome instability assessment	benzene	total number of normochromatic erythrocytes, bone marrow, post-exposure (x 10³) [n] bm_NCE_total_post 112437 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=142

French1:121	French1	chromosome instability assessment	benzene	percentage of red blood cells that are reticulocytes, bone marrow, post-exposure [%] bm_RET_pct_post 114401 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=142

French1:129	French1	chromosome instability assessment	benzene	number of non-micronucleated reticulocytes, bone marrow, post-exposure (x 10³) [n] bm_RET_post 114409 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=142

French1:133	French1	chromosome instability assessment	benzene	number of micronucleated reticulocytes, bone marrow, post-exposure [n] bm_RET_MN_post 114413 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=142

French1:137	French1	chromosome instability assessment	benzene	number of micronucleated reticulocytes, bone marrow per 1000 reticulocytes, post-exposure [n] bm_RET_MN_post_adj 114417 group: 0 ppm 1 ppm 10 ppm 100 ppm	DO population	m	5-8wks	N=142

French1: Effects of benzene inhalation exposure in male Diversity Outbred (DO) mice (2015) [Pending]