Acclimation and testing periods
Ages Mice were housed in JAX® production rooms until either 4 wks of age Feed NIH31 diet containing 6% fat, 17% protein, plus vitamin and mineral fortification (Purina Mills International, Brentwood, MO). The full composition of this open formula diet is available at here Water Animal were provided free access to acidified water (pH 2.5 with HCl to retard bacterial growth) HousingThe mice were received into our research colony at 4 weeks of age, and housed in groups of 3-4 of the same sex Bedding Sterilized White Pine shavings Health status Sentinel mice were periodically examined throughout the study and were free of microbiological infections