Donahue16 animal documentation

Bone mineral density and body composition in C57BL/6J-Chr#^A/J/NaJ mouse chromosome substitution strains   (2012)

Acclimation and testing periods

Vendor	Mice were obtained from the Repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)
Ages	Mice were received at 4-5 wks of age and tested 24-29 wks of age
Feed	Mice were fed pelleted and autoclaved 5K52 diet, modified from the NIH 31M open formula with 6% fat (LabDiet® 5K52 6% Fat, St. Louis, MO)
Water	Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)
Housing	Mice were housed in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding	Autoclaved pine shavings (Crobb Box, Ellsworth, ME)
Humidity	Relative humidity of 35% ± 4%
Photoperiod	12-h light: 12-h dark cycle
Temperature	22 ± 2°C
Regulatory compliance	All procedures were conducted in strict compliance with the policies on animal welfare of the National Institutes of Health and were approved by the Animal Care and Use Committee of The Jackson Laboratory