Donahue13 animal documentation

Reproductive performance of 13 CXB recombinant inbred strains of mice   (2011)

Acclimation and testing periods

Supplier	CXB recombinant inbred mice were obtained from the repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)
Ages	All mice were pair-mated to siblings between 3 to 16 wks of age, and retired from breeding between 26 to 50 wks of age
Feed	Pelleted and autoclaved 5K52 diet, modified from the NIH 31M open formula with 6% fat (LabDiet® 5K52 6% Fat, St. Louis, MO), was provided ad libitum
Water	Access to autoclaved acidified water (pH 2.8 to 3.1) was provided ad libitum
Housing	Mice were housed in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF barrier room. The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding	Autoclaved pine shavings (Crobb Box, Ellsworth, ME)
Humidity	35 ± 4%
Photoperiod	12-h light: 12-h dark cycle
Temperature	22 ± 2°C
Regulatory compliance	All procedures were conducted in strict compliance with the policies on animal welfare of the National Institutes of Health and were approved by the Animal Care and Use Committee of The Jackson Laboratory