Animal documentation
Source The Jackson Laboratory (Bar Harbor, ME) Ages 4 wks of age at shipping; 12-16 wks of age at testing
FeedNIH31 5K52 (LabDiet/PMI Nutrition, St. Louis MO), provided ad libitum
Water Provided ad libitum Temperature Not specified Humidity Not specified Housing Duplex polycarbonate cages on ventilated racks which provided 99.997% HEPA-filtered air to each cage; lid of each cage was fitted with a filtered top which reduced cross-cage odor exposure when mice were removed from the ventilated racks for testing; bedding changed weekly; nestlets and Shepherd Shacks were provided for enrichment; housed 3-5 per cagePhotoperiod 12h:12h light-dark cycle (lights on at 0600h; novelty testing conducted between 1000 and 1400h; IVSA testing began at 0900h) Care and UseAll procedures approved by The Jackson Laboratory Animal Care and Use Committee and conducted in compliance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals.