MPD: Data set: Chesler6

Chesler6: Quantitative trait loci for sensitivity to ethanol intoxication in a C57BL/6J x 129S1/SvImJ inbred mouse cross (2012)

Chesler EJ, Plitt A, Fisher D, Hurd B, Lederle L, Bubier JA, Kiselycznyk C, Holmes A. Quantitative trait loci for sensitivity to ethanol intoxication in a C57BL/6J × 129S1/SvImJ inbred mouse cross. Mamm Genome. 2012 Jun;23(5-6):305-2Epub 2012 Feb 28. PubMed 22371272

Chesler6 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.

Ontology terms mapped to Chesler6 measures:

MA:0000059 blood
MP:0001259 abnormal body weight
MP:0001516 abnormal motor coordination/balance
MP:0001961 abnormal reflex
MP:0003492 abnormal involuntary movement
MP:0005443 abnormal ethanol metabolism
MP:0005535 abnormal body temperature
MP:0020157 abnormal behavioral response to alcohol
VT:0001259 body mass
VT:0001516 motor coordination/balance trait
VT:0001961 reflex trait
VT:0003492 involuntary movement trait
VT:0005443 ethanol metabolism trait
VT:0005535 body temperature trait
VT:0010489 response to alcohol trait
VT:0015078 blood homeostasis trait

Investigators	Elissa J Chesler The Jackson Laboratory, Bar Harbor, ME
Participants	Plitt A, Fisher D, Hurd B, Lederle L, Bubier JA, Kiselycznyk C, Holmes A
Contact	Elissa J Chesler elissa.chesler@jax.org
Affiliated Center	QTL Archive
Acknowledgements	Research supported by the National Institute of Alcohol Abuse and Alcoholism Intramural Research Program (Z01 AA000411). EJC is supported by The Jackson Laboratory and R01 AA18776.
Project type	QTL Archive data set
MPD identifiers	Chesler6 MPD:271
Data changelog	No updates/corrections. Initial release date: 03/2012.

Individual variation in sensitivity to acute ethanol (EtOH) challenge is associated with alcohol drinking and abuse. Inbred strain C57BL/6J (B6) and 129S1/SvImJ (S1) showed different responses on certain measures of acute EtOH intoxication. To gain insight into genetic factors contributing to these differences, a QTL analysis of EtOH traits was performed in B6x129S1 F2 population. The result revealed several additive and interacting loci, and the QTLs for different EtOH phenotypes were largely non-overlapping. For detailed protocols, see Chesler et al 2012.

QTL data set parameters:

Progenitor strains	129S1/SvImJ C57BL/6J
Cross type	F2 reciprocal intercross
Population size	178 ♀ 195 ♂
Notes	one mouse was missing sex infomation
Age	9-34wks

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Chesler6	body weight	(none)	mouse body weight [g] B_wght 27101	QTL population	both	9-34wks	N=186

Chesler6	rotarod	(none)	latency to fall, initial trial [s] Rotarod_base 27102	QTL population	both	9-34wks	N=186

Chesler6	rotarod	(none)	latency difference, final minus initial trial latency to fall on rotarod through 10 training trials [s] Rotarod_learn 27103	QTL population	both	9-34wks	N=186

Chesler6	rotarod	ethanol	latency difference, two trial post alcohol average minus pre trial latency to fall off rotarod [s] Rotarod_atax 27104	QTL population	both	9-34wks	N=186

Chesler6	body temperature	ethanol	post alcohol minus pre alcohol body temperature [° C] Hypotherm 27105	QTL population	both	9-34wks	N=186

Chesler6	behavior observation	ethanol	alcohol induced loss of righting reflex, latency to return of reflex [s] LORR 27106	QTL population	both	9-34wks	N=186

Chesler6	alcohol quantification	ethanol	blood ethanol concentration at return of righting reflex [mg/mL] LORR_BEC 27107	QTL population	both	9-34wks	N=186

Chesler6	rotarod	(none)	average latency to fall over 10 training trials [second] Avg1to10 27108	QTL population	both	9-34wks	N=186

Chesler6	rotarod	ethanol	latency to fall, first alcohol trial [s] RotaTest1 27109	QTL population	both	9-34wks	N=186

Associated genomic features

MGI-curated QTL / genomic features that have been associated with this data set:
Eih3 Eih4 Eih5 Elorr3

Project / data set: Chesler6 a.k.a. Chesler_2012
Excel file: Data_Description_B6x129S_Chesler2012.xlsx
CSV file: Chesler2012_B6x129S_B37_Data.csv
Readme / text file: readme_Chesler2012.txt
QC plots, figures, etc.: Chr12RFplot_Chesler2012.png
QC plots, figures, etc.: MapComparison_Chesler2012.png
QC plots, figures, etc.: Mapplot_Chesler2012.png
QC plots, figures, etc.: MissingGeno_Chesler2012.png
QC plots, figures, etc.: RFplot_Chesler2012.png