MPD: Data set: Berndt2

Berndt A, Leme AS, Williams LK, Von Smith R, Savage HS, Stearns TM, Tsaih SW, Shapiro SD, Peters LL, Paigen B, Svenson KL. Comparison of unrestrained plethysmography and forced oscillation for identifying genetic variability of airway responsiveness in inbred mice. Physiol Genomics. 2011 Jan 7;43(1):1-1Epub 2010 Sep 7. PubMed 20823217 FullText

Berndt2 downloads
• Download Berndt2 project data set animal data, as uploaded
• Download Berndt2 animal data matrix with factor-related expansion applied as necessary
• Download Berndt2 strain means, SD, N, etc. one row per strain/sex/measure
• Berndt2 supplementary data

Ontology terms mapped to Berndt2 measures:

MA:0000415 lung
MP:0001951 abnormal breathing pattern
MP:0002313 abnormal tidal volume
MP:0002328 abnormal airway resistance
MP:0002334 abnormal airway responsiveness
MP:0008872 abnormal physiological response to xenobiotic
MP:0011043 abnormal lung elastance
VT:0001942 lung capacity
VT:0001943 respiration trait
VT:0002314 respiratory mechanics trait
VT:0002338 pulmonary ventilation trait
VT:0010487 response to xenobiotic stimulus trait

Investigators	Annerose Berndt University of Pittsburgh School of Medicine, Pittsburgh, PA Adriana S Leme University of Pittsburgh School of Medicine, Pittsburgh, PA Beverly Paigen The Jackson Laboratory, Bar Harbor, ME Steven D Shapiro University of Pittsburgh School of Medicine, Pittsburgh, PA Karen L Svenson The Jackson Laboratory, Bar Harbor, ME
Contact	Annerose Berndt anb128@pitt.edu Lab web site
Acknowledgements	Funding provided by HL66611, HL83069, CA34196
Project type	Phenotype strain survey data set
MPD identifiers	Berndt2 MPD:351
Data changelog	1 update/correction. Initial release date: 03/2011.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice were assessed for respiratory parameters at baseline and then again after methacholine (MCh) challenge using two different techniques in two cohorts: 1) unrestrained plethysmograph technique (BUXCO), given MCh doses of 5, 10, and 20 mg/mL; 2) anesthetized forced oscillation technique (FlexiVent), given MCh doses of 1, 3, 10, and 30 mg/mL . The "MCh response index" is the average slope of the MCh challenge series (see protocol for details).

Procedures conducted:

• plethysmography		Lung, respiratory function. Baseline vs. methacholine-challenged (several doses).
• ventilator		Lung, respiratory function. Anesthetized forced oscillation. Baseline vs. methacholine-challenged (several doses).

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Berndt2:1	Berndt2	plethysmography	methacholine	minute ventilation [mL/min] min_vent 35101 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:8	Berndt2	plethysmography	methacholine	breath frequency [n/min] breath_freq 35108 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:14	Berndt2	plethysmography	methacholine	inspiratory phase [s] inspiratory 35114 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:21	Berndt2	plethysmography	methacholine	expiratory phase [s] expiratory 35121 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:28	Berndt2	plethysmography	methacholine	respiratory cycle [s] tot_respi 35128 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:34	Berndt2	plethysmography	methacholine	duty cycle [ratio] duty_cycle 35134 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:41	Berndt2	plethysmography	methacholine	inspiratory flow rate [mL/kg/s] insp_flow 35141 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:48	Berndt2	plethysmography	methacholine	expiratory flow rate [mL/kg/s] exp_flow 35148 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:54	Berndt2	plethysmography	methacholine	respiratory flow rate [mL/kg/s] resp_flow 35154 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:61	Berndt2	plethysmography	methacholine	enhanced pause penh 35161 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:68	Berndt2	plethysmography	methacholine	tidal volume [mL] tidal_vol 35168 saline 5 MCh 10 MCh 20 MCh response index	inbred	29	both	9-12wks	N=8

Berndt2:81	Berndt2	ventilator	methacholine	airway resistance (H₂0 pressure) [cm/mL/s] airway_res 35181 saline 1 MCh 3 MCh 10 MCh 30 MCh response index	inbred	29	both	9-12wks	N=9

Berndt2:91	Berndt2	ventilator	methacholine	lung tissue dynamic elastance (H₂0 pressure) [cm/mL] airway_elas 35191 saline 1 MCh 3 MCh 10 MCh 30 MCh response index	inbred	29	both	9-12wks	N=9

Berndt2: Unrestrained plethysmograph and anesthetized forced oscillation methods of measuring lung function in 29 inbred strains of mice (2010)