ITP phenotypes:   rotarod     acarbose

Trial code: ACA_mid Dose: 1000 ppm   initiated at age: 8 mo Sex: f Cohort: C2013
Trial code: ACA_mid Dose: 1000 ppm   initiated at age: 8 mo Sex: m Cohort: C2013
The plots below show the means with ± SEM error bars





Note: mice were tested at 4 mo (young group) or 22 mo (old groups).

ANOVA

Factor Df Sum Sq Mean Sq F value Pr(>F)
group 2 91619.1569 45809.5784 23.7802 < 0.0001
time 5 153204.8034 30640.9607 15.906 < 0.0001
group:time 10 26383.3358 2638.3336 1.3696 0.1918
Residuals 419 807151.5833 1926.3761 NA NA


Means

group time mean SE
Old_ACA_mid time_d1 25.1827 2.8956
Old_ACA_mid time_d2 46.9815 5.4483
Old_ACA_mid time_d3 62.1469 6.2446
Old_ACA_mid time_d4 68.863 7.169
Old_ACA_mid time_d5 91.9667 8.9364
Old_ACA_mid time_d6 106.063 9.4955
Old_Control time_d1 21.8235 5.2001
Old_Control time_d2 30.0705 6.6877
Old_Control time_d3 39.1346 10.0359
Old_Control time_d4 45.5704 10.9757
Old_Control time_d5 51.4086 10.6497
Old_Control time_d6 51.3222 10.6327
Young_Control time_d1 37.4193 6.7633
Young_Control time_d2 59.7807 10.7304
Young_Control time_d3 77.1789 10.7705
Young_Control time_d4 82.3421 11.6971
Young_Control time_d5 97.0263 13.3159
Young_Control time_d6 85.8895 9.8496


Pairwise comparisons (Tukey multiple-test correction)

contrast time estimate SE df t.ratio p.value
Old_ACA_mid - Old_Control time_d1 3.3593 11.9455 419 0.2812 0.9573
Old_ACA_mid - Young_Control time_d1 -12.2366 13.1429 419 -0.931 0.621
Old_Control - Young_Control time_d1 -15.5958 13.1429 419 -1.1866 0.4618
Old_ACA_mid - Old_Control time_d2 16.911 12.0598 419 1.4023 0.3406
Old_ACA_mid - Young_Control time_d2 -12.7992 13.1429 419 -0.9739 0.5938
Old_Control - Young_Control time_d2 -29.7102 13.2469 419 -2.2428 0.0653
Old_ACA_mid - Old_Control time_d3 23.0123 11.9455 419 1.9264 0.1325
Old_ACA_mid - Young_Control time_d3 -15.032 13.1429 419 -1.1437 0.4877
Old_Control - Young_Control time_d3 -38.0444 13.1429 419 -2.8947 0.0111
Old_ACA_mid - Old_Control time_d4 23.2926 11.9455 419 1.9499 0.1262
Old_ACA_mid - Young_Control time_d4 -13.4791 13.1429 419 -1.0256 0.5611
Old_Control - Young_Control time_d4 -36.7717 13.1429 419 -2.7978 0.0148
Old_ACA_mid - Old_Control time_d5 40.558 11.9455 419 3.3953 0.0022
Old_ACA_mid - Young_Control time_d5 -5.0596 13.1429 419 -0.385 0.9216
Old_Control - Young_Control time_d5 -45.6177 13.1429 419 -3.4709 0.0017
Old_ACA_mid - Old_Control time_d6 54.7407 11.9455 419 4.5825 < 0.0001
Old_ACA_mid - Young_Control time_d6 20.1735 13.1429 419 1.5349 0.2756
Old_Control - Young_Control time_d6 -34.5673 13.1429 419 -2.6301 0.024










Note: mice were tested at 4 mo (young group) or 22 mo (old groups).

ANOVA

Factor Df Sum Sq Mean Sq F value Pr(>F)
group 2 32395.2344 16197.6172 17.8785 < 0.0001
time 5 73792.508 14758.5016 16.2901 < 0.0001
group:time 10 850.492 85.0492 0.0939 0.9999
Residuals 360 326153.4663 905.9819 NA NA


Means

group time mean SE
Old_ACA_mid time_d1 20.5977 3.7788
Old_ACA_mid time_d2 31.1747 4.682
Old_ACA_mid time_d3 43.458 6.2622
Old_ACA_mid time_d4 48.6782 5.6369
Old_ACA_mid time_d5 57.3805 7.7317
Old_ACA_mid time_d6 61.4621 7.7832
Old_Control time_d1 17.9619 3.8359
Old_Control time_d2 23.7714 4.2236
Old_Control time_d3 30.5345 4.7249
Old_Control time_d4 38.6048 5.3548
Old_Control time_d5 50.519 5.7001
Old_Control time_d6 53.6619 5.1716
Young_Control time_d1 37.05 4.8336
Young_Control time_d2 45.6283 5.7673
Young_Control time_d3 61.6967 7.4781
Young_Control time_d4 64.175 7.3345
Young_Control time_d5 74.7533 8.5563
Young_Control time_d6 76.4583 8.0075


Pairwise comparisons (Tukey multiple-test correction)

contrast time estimate SE df t.ratio p.value
Old_ACA_mid - Old_Control time_d1 2.6358 9.7956 360 0.2691 0.9609
Old_ACA_mid - Young_Control time_d1 -16.4523 8.7487 360 -1.8805 0.1458
Old_Control - Young_Control time_d1 -19.0881 10.4887 360 -1.8199 0.1645
Old_ACA_mid - Old_Control time_d2 7.4033 9.7956 360 0.7558 0.7303
Old_ACA_mid - Young_Control time_d2 -14.4536 8.7487 360 -1.6521 0.2253
Old_Control - Young_Control time_d2 -21.8569 10.4887 360 -2.0839 0.0946
Old_ACA_mid - Old_Control time_d3 12.9235 9.7956 360 1.3193 0.3853
Old_ACA_mid - Young_Control time_d3 -18.2386 8.7487 360 -2.0847 0.0944
Old_Control - Young_Control time_d3 -31.1621 10.4887 360 -2.971 0.0089
Old_ACA_mid - Old_Control time_d4 10.0734 9.7956 360 1.0284 0.5594
Old_ACA_mid - Young_Control time_d4 -15.4968 8.7487 360 -1.7713 0.1807
Old_Control - Young_Control time_d4 -25.5702 10.4887 360 -2.4379 0.0403
Old_ACA_mid - Old_Control time_d5 6.8614 9.7956 360 0.7005 0.7634
Old_ACA_mid - Young_Control time_d5 -17.3729 8.7487 360 -1.9858 0.1172
Old_Control - Young_Control time_d5 -24.2343 10.4887 360 -2.3105 0.0556
Old_ACA_mid - Old_Control time_d6 7.8002 9.7956 360 0.7963 0.7055
Old_ACA_mid - Young_Control time_d6 -14.9963 8.7487 360 -1.7141 0.2013
Old_Control - Young_Control time_d6 -22.7964 10.4887 360 -2.1734 0.0772










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