MPD: ITP phenotypes: fat pads acarbose

ITP phenotypes: fat pads acarbose

Trial code: ACA_mid

Dose: 1000 ppm initiated at age: 4 mo

Sex: both

Cohort: C2013

Weight of fat pads (4 types) vs. body weight, for a subset of mice at 12 mo of age

Statistical definitions

Subscapular fat pads

ANCOVA analysis

Term	Df	Sum Sq	Mean Sq	F value	Pr(>F)
Sex	1	0.0391	0.0391	11.3906	0.0022
Group	1	0.0018	0.0018	0.5118	0.4803
BW	1	0.6415	0.6415	186.8221	< 0.0001
Sex:Group	1	0.0298	0.0298	8.683	0.0064
Residuals	28	0.0961	0.0034	NA	NA

Means

Sex	Group	Mean	SE
f	ACA_mid	0.4117	0.0447
m	ACA_mid	0.3369	0.0327
f	Control	0.2636	0.0412
m	Control	0.4865	0.0673

Pairwise comparisons (Tukey multiple-test correction)

contrast	estimate	SE	df	t.ratio	p.value
f,ACA_mid - m,ACA_mid	0.258562607047098	0.0323610320130506	28	7.98993700024229	0.00000006190932444472
f,ACA_mid - f,Control	0.0272032276911691	0.031968096101791	28	0.850949259053467	0.829632182101246
f,ACA_mid - m,Control	0.157852373408822	0.0344916221896714	28	4.57654245836229	0.000486107563800076
m,ACA_mid - f,Control	-0.231359379355929	0.039010457263762	28	-5.93070154988533	0.000012619068811448
m,ACA_mid - m,Control	-0.100710233638276	0.027923653024659	28	-3.60662817108278	0.00618782558097564
f,Control - m,Control	0.130649145717653	0.0417961077142391	28	3.12586871990339	0.0201887712846476

Mesenteric fat pads

ANCOVA analysis

Term	Df	Sum Sq	Mean Sq	F value	Pr(>F)
Sex	1	0.154	0.154	43.7368	< 0.0001
Group	1	0.0386	0.0386	10.967	0.0025
BW	1	0.5135	0.5135	145.8105	< 0.0001
Residuals	29	0.1021	0.0035	NA	NA

Means

Sex	Group	Mean	SE
f	ACA_mid	0.2262	0.0365
m	ACA_mid	0.2582	0.0342
f	Control	0.1779	0.0414
m	Control	0.4236	0.0637

Pairwise comparisons (Tukey multiple-test correction)

contrast	estimate	SE	df	t.ratio	p.value
f - m	0.1219	0.0297	29	4.1007	0.0003
ACA_mid - Control	-0.0957	0.0208	29	-4.5981	0.0001

Gonadal fat pads

ANCOVA analysis

Term	Df	Sum Sq	Mean Sq	F value	Pr(>F)
Sex	1	1.5525	1.5525	29.1469	< 0.0001
Group	1	0.0177	0.0177	0.3318	0.569
BW	1	6.3626	6.3626	119.453	< 0.0001
Residuals	29	1.5447	0.0533	NA	NA

Means

Sex	Group	Mean	SE
f	ACA_mid	0.8229	0.1685
m	ACA_mid	0.9519	0.1494
f	Control	0.5232	0.1374
m	Control	1.2853	0.2075

Pairwise comparisons (Tukey multiple-test correction)

contrast	estimate	SE	df	t.ratio	p.value
f - m	0.4698	0.1156	29	4.0653	0.0003
ACA_mid - Control	-0.142	0.0809	29	-1.7547	0.0899

Inguinal fat pads

ANCOVA analysis

Term	Df	Sum Sq	Mean Sq	F value	Pr(>F)
Sex	1	0.184	0.184	20.7414	0.0001
BW	1	1.0053	1.0053	113.3356	< 0.0001
Residuals	30	0.2661	0.0089	NA	NA

Means

Sex	Group	Mean	SE
f	ACA_mid	0.3763	0.0482
m	ACA_mid	0.4041	0.0604
f	Control	0.2296	0.0446
m	Control	0.5114	0.0959

Pairwise comparisons (Tukey multiple-test correction)

contrast	estimate	SE	df	t.ratio	p.value
f - m	0.206	0.0469	30	4.3894	0.0001

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